WB (Western Blot)
(Detection of human and mouse NMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody AAA212573 (lot AAA212573-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse NMT1 Polyclonal Antibody | anti-NMT1 antibody
Rabbit anti-NMT1 Antibody, Affinity Purified
Gene Names
NMT1; NMT
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NMT1, Antibody; Rabbit anti-NMT1 Antibody, Affinity Purified; NMT; Peptide N-myristoyltransferase 1; type I N-myristoyltransferase; NMT 1; myristoyl-CoA:protein N-myristoyltransferase 1; long form, NMT-L; alternative, short form NMT-S; glycylpeptide N-tetradecanoyltransferase 1; Glycylpeptide N-tetradecanoyltransferase 1; N-myristoyltransferase 1; anti-NMT1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NMT1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody AAA212573 (lot AAA212573-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse NMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody AAA212573 (lot AAA212573-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 to 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody AAA212573 (lot AAA212573-1) used for IP at 6 ug per reaction. NMT1 was also immunoprecipitated by rabbit anti-NMT1 antibody For blotting immunoprecipitated NMT1, AAA212573 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human NMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 to 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody AAA212573 (lot AAA212573-1) used for IP at 6 ug per reaction. NMT1 was also immunoprecipitated by rabbit anti-NMT1 antibody For blotting immunoprecipitated NMT1, AAA212573 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-NMT1 antibody
NMT1 adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P30419].
Product Categories/Family for anti-NMT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,141 Da
NCBI Official Full Name
glycylpeptide N-tetradecanoyltransferase 1
NCBI Official Synonym Full Names
N-myristoyltransferase 1
NCBI Official Symbol
NMT1
NCBI Official Synonym Symbols
NMT
NCBI Protein Information
glycylpeptide N-tetradecanoyltransferase 1
UniProt Protein Name
Glycylpeptide N-tetradecanoyltransferase 1
UniProt Gene Name
NMT1
UniProt Synonym Gene Names
NMT; NMT 1; Type I N-myristoyltransferase
UniProt Entry Name
NMT1_HUMAN
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Product Notes
The NMT1 nmt1 (Catalog #AAA212573) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NMT1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NMT1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NMT1 nmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NMT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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