IF (Immunofluorescence)
(AAA328982 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328982 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit Nociceptin Polyclonal Antibody | anti-PNOC antibody
Nociceptin Antibody
Gene Names
PNOC; NOP; OFQ; N/OFQ; PPNOC; ppN/OFQ
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Nociceptin, Antibody; Nociceptin Antibody; N/OFQ; N23K; N23K/N27K; Nociceptin; included; nocistatin; Npnc1; OFQ; OFQ/N; Orphanin FQ; Orphanin FQ2; Pnoc; PNOC_HUMAN; ppN/OFQ; PPNOC; pre-pro-N/OFQ; prepronociceptin; pronociceptin; Propronociceptin; anti-PNOC antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Nociceptin Antibody detects endogenous levels of total Nociceptin.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
176
Applicable Applications for anti-PNOC antibody
ELISA
Immunogen
A synthesized peptide derived from human Nociceptin, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328982 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328982 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328982 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328982 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from Rat brain, using Nociceptin Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from Rat brain, using Nociceptin Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from Myeloma cells, using Nociceptin Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from Myeloma cells, using Nociceptin Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)
Related Product Information for anti-PNOC antibody
Function: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.Has potent analgesic activity.
Post Translational Modifications: Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Subcellular Location: Secreted.
Tissue Specificity: Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
Similarity: Belongs to the opioid neuropeptide precursor family.
Post Translational Modifications: Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Subcellular Location: Secreted.
Tissue Specificity: Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
Similarity: Belongs to the opioid neuropeptide precursor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)20kD, 25kD.
Predicted Molecular Weight: (Calculated)20kDa.
Predicted Molecular Weight: (Calculated)20kDa.
NCBI Official Full Name
Prepronociceptin
NCBI Official Synonym Full Names
prepronociceptin
NCBI Official Symbol
PNOC
NCBI Official Synonym Symbols
NOP; OFQ; N/OFQ; PPNOC; ppN/OFQ
NCBI Protein Information
prepronociceptin
UniProt Protein Name
Prepronociceptin
UniProt Gene Name
PNOC
UniProt Synonym Gene Names
OFQ
UniProt Entry Name
PNOC_HUMAN
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Product Notes
The PNOC pnoc (Catalog #AAA328982) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nociceptin Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Nociceptin can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the PNOC pnoc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nociceptin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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