IF (Immunofluorescence)
(AAA31099 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31099) and mouse antibeta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue))
Rabbit NOD2 Polyclonal Antibody | anti-NOD2 antibody
NOD2 Antibody
Gene Names
NOD2; CD; ACUG; BLAU; IBD1; YAOS; BLAUS; NLRC2; NOD2B; CARD15; CLR16.3; PSORAS1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific)
Synonyms
NOD2, Antibody; NOD2 Antibody; ACUG; Arthrocutaneouveal granulomatosis; BLAU; CARD15; Caspase recruitment domain family, member 15; Caspase recruitment domain protein 15; Caspase recruitment domain-containing protein 15; CD; CLR16.3; IBD1; Inflammatory bowel disease protein 1; LRR containing protein; NLR family, CARD domain containing 2; NLRC2; NOD like receptor C2; NOD2; NOD2 protein; NOD2_HUMAN; NOD2B; nucleotide binding oligomerization domain 2; Nucleotide binding oligomerization domain containing 2; Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2; Nucleotide-binding oligomerization domain-containing protein 2; PSORAS1; anti-NOD2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific)
Form/Format
Liquid. IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
~1mg/ml (varies by lot)
Sequence Length
1013
Applicable Applications for anti-NOD2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB 1:500-1:2000
IHC 1:50-1:200
IF/ICC 1:100-1:500
The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC 1:50-1:200
IF/ICC 1:100-1:500
The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human NOD2, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA31099 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31099) and mouse antibeta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue))
IF (Immunofluorescence)
(AAA31099 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31099) and mouse antibeta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue))
WB (Western Blot)
(Western blot analysis of extracts from various samples, using NOD2 antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Mouse Myeloma cell; Lane 4: mouse lung;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using NOD2 antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Mouse Myeloma cell; Lane 4: mouse lung;)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
100-110 kDa
NCBI Official Full Name
nucleotide-binding oligomerization domain-containing protein 2 isoform 2
NCBI Official Synonym Full Names
nucleotide binding oligomerization domain containing 2
NCBI Official Symbol
NOD2
NCBI Official Synonym Symbols
CD; ACUG; BLAU; IBD1; YAOS; BLAUS; NLRC2; NOD2B; CARD15; CLR16.3; PSORAS1
NCBI Protein Information
nucleotide-binding oligomerization domain-containing protein 2
UniProt Protein Name
Nucleotide-binding oligomerization domain-containing protein 2
UniProt Gene Name
NOD2
UniProt Synonym Gene Names
CARD15; IBD1
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Product Notes
The NOD2 nod2 (Catalog #AAA31099) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOD2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NOD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500 The optimal dilution should be determined by the end user. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the NOD2 nod2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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