WB (Western Blot)
(Anti-NOG rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:33 kDa)
Rabbit Noggin/NOG Polyclonal Antibody | anti-NOG antibody
Anti-Noggin/NOG Antibody, Rabbit Polyclonal
Gene Names
NOG; SYM1; SYNS1; SYNS1A
Reactivity
HumanPredicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Noggin/NOG, Antibody; Anti-Noggin/NOG Antibody, Rabbit Polyclonal; NOG Antibody, Rabbit PAb, Antigen Affinity Purified; noggin; Anti-Noggin Antibody; Anti-SYM1 Antibody; Anti-SYNS1 Antibody; anti-NOG antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Noggin/NOG
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NOG antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the N-terminus of the human NOG
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the human NOG, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-NOG rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:33 kDa)
WB (Western Blot)
(Anti-NOG rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:33 kDa)
IP (Immunoprecipitation)
(NOG was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate0.5 uL anti-NOG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NOG rabbit polyclonal antibody,at 1:350 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 33 kDa)
IP (Immunoprecipitation)
(NOG was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate0.5 uL anti-NOG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NOG rabbit polyclonal antibody,at 1:350 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 33 kDa)
Related Product Information for anti-NOG antibody
Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities, with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs, Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7 and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both types I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes, including embryonic dorsal-ventral axis, induction of neural tissue, the formation of joints in the skeletal system, and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-beta signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion.
References
Zimmerman LB, et al. (1996) The Spemann organizer signal noggin binds and inactivates bone morphogenetic protein 4. Cell. 86(4): 599-606.Chandramore K, et al. (2010) Cloning of noggin gene from hydra and analysis of its functional conservation using Xenopus laevis embryos. Evol Dev. 12(3): 267-74.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
noggin
NCBI Official Synonym Full Names
noggin
NCBI Official Symbol
NOG
NCBI Official Synonym Symbols
SYM1; SYNS1; SYNS1A
NCBI Protein Information
noggin
UniProt Protein Name
Noggin
UniProt Gene Name
NOG
UniProt Entry Name
NOGG_HUMAN
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Product Notes
The NOG nog (Catalog #AAA254196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Noggin/NOG Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Noggin/NOG can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NOG nog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Noggin/NOG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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