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IHC (Immunohistochemistry)
(AAA329118 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit Nogo Receptor Polyclonal Antibody | anti-RTN4R antibody
Nogo Receptor Antibody
Gene Names
RTN4R; NGR; NOGOR
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(88%), Horse(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Nogo Receptor, Antibody; Nogo Receptor Antibody; NgR; Nogo 66 receptor; Nogo receptor; Nogor; Reticulon 4 receptor; Rtn4r; UNQ330/PRO526; anti-RTN4R antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Horse(88%)
Predicted Reactivity: Pig(88%), Horse(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Nogo Receptor Antibody detects endogenous levels of total Nogo Receptor.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
473
Applicable Applications for anti-RTN4R antibody
ELISA
Immunogen
A synthesized peptide derived from human Nogo Receptor, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(AAA329118 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA329118 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA329118 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA329118 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from PC12, using Nogo Receptor Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from PC12, using Nogo Receptor Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using Nogo Receptor Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using Nogo Receptor Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-RTN4R antibody
Function: Receptor for RTN4, OMG and MAG (PubMed:12037567, PubMed:12068310, PubMed:12426574, PubMed:12089450, PubMed:16712417, PubMed:18411262, PubMed:12839991, PubMed:19052207). Functions as receptor for the sialylated gangliosides GT1b and GM1 (PubMed:18411262). Besides, functions as receptor for chondroitin sulfate proteoglycans (By similarity). Can also bind heparin (By similarity). Intracellular signaling cascades are triggered via the coreceptor NGFR (PubMed:12426574). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:16712417, PubMed:22325200). Mediates axonal growth inhibition (PubMed:12839991, PubMed:19052207, PubMed:28892071). Plays a role in regulating axon regeneration and neuronal plasticity in the adult central nervous system. Plays a role in postnatal brain development. Required for normal axon migration across the brain midline and normal formation of the corpus callosum. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated via interaction with MAG. Acts in conjunction with RTN4 and LINGO1 in regulating neuronal precursor cell motility during cortical development. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200).
Post Translational Modifications: N-glycosylated. O-glycosylated. Contains terminal sialic acid groups on its glycan chains.
Subcellular Location: Cell membrane>Lipid-anchor. Membrane raft. Cell projection>Dendrite. Cell projection>Axon. Perikaryon. Note: Detected along dendrites and axons, close to synapses, but clearly excluded from synapses.
Tissue Specificity: Widespread in the brain but highest levels in the gray matter. Low levels in heart and kidney; not expressed in oligodendrocytes (white matter).
Subunit Structure: Homodimer (PubMed:18411262). Interacts with MAG (PubMed:12089450, PubMed:12839991, PubMed:18411262, PubMed:19052207). Interacts with RTN4 (PubMed:12839991, PubMed:19052207). Interacts with NGFR (PubMed:12426574, PubMed:18411262, PubMed:19052207). Interacts with LINGO1 (PubMed:14966521, PubMed:19052207). Interacts with KIAA0319L (PubMed:20697954). Interacts with OLFM1; this inhibits interaction with LINGO1 and NGFR (By similarity). Interacts with OMG (PubMed:12068310, PubMed:12839991, PubMed:19052207).
Similarity: Belongs to the Nogo receptor family.
Post Translational Modifications: N-glycosylated. O-glycosylated. Contains terminal sialic acid groups on its glycan chains.
Subcellular Location: Cell membrane>Lipid-anchor. Membrane raft. Cell projection>Dendrite. Cell projection>Axon. Perikaryon. Note: Detected along dendrites and axons, close to synapses, but clearly excluded from synapses.
Tissue Specificity: Widespread in the brain but highest levels in the gray matter. Low levels in heart and kidney; not expressed in oligodendrocytes (white matter).
Subunit Structure: Homodimer (PubMed:18411262). Interacts with MAG (PubMed:12089450, PubMed:12839991, PubMed:18411262, PubMed:19052207). Interacts with RTN4 (PubMed:12839991, PubMed:19052207). Interacts with NGFR (PubMed:12426574, PubMed:18411262, PubMed:19052207). Interacts with LINGO1 (PubMed:14966521, PubMed:19052207). Interacts with KIAA0319L (PubMed:20697954). Interacts with OLFM1; this inhibits interaction with LINGO1 and NGFR (By similarity). Interacts with OMG (PubMed:12068310, PubMed:12839991, PubMed:19052207).
Similarity: Belongs to the Nogo receptor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)50kD.
Predicted Molecular Weight: (Calculated)51kDa.
Predicted Molecular Weight: (Calculated)51kDa.
NCBI Official Full Name
Reticulon-4 receptor
NCBI Official Synonym Full Names
reticulon 4 receptor
NCBI Official Symbol
RTN4R
NCBI Official Synonym Symbols
NGR; NOGOR
NCBI Protein Information
reticulon-4 receptor
UniProt Protein Name
Reticulon-4 receptor
UniProt Gene Name
RTN4R
UniProt Synonym Gene Names
NOGOR; NgR
UniProt Entry Name
RTN4R_HUMAN
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Product Notes
The RTN4R rtn4r (Catalog #AAA329118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nogo Receptor Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(88%), Horse(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's Nogo Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the RTN4R rtn4r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nogo Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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