WB (Western Blot)
(Anti-NOX1 antibody, AAA44951, Western blottingAll lanes: Anti NOX1 (AAA44951) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 65KDObserved bind size: 65KD)
Rabbit anti-Human NOX1 Polyclonal Antibody | anti-NOX1 antibody
Anti-NOX1 antibody
Gene Names
NOX1; MOX1; NOH1; NOH-1; GP91-2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
NOX1, Antibody; Anti-NOX1 antibody; NADPH oxidase 1(NOX-1); NADPH oxidase 1; GP91 2 antibody; Mitogenic oxidase(pyridine nucleotide dependent superoxide generating) antibody; Mitogenic oxidase 1 antibody; MOX-1 antibody; MOX1 antibody; NADH/NADPH mitogenic oxidase subunit P65 MOX antibody; NADH/NADPH mitogenic oxidase subunit P65-MOX antibody; NADPH oxidase 1 antibody; NADPH oxidase 1 variant NOH 1L antibody; NADPH oxidase homolog 1 antibody; NOH 1 antibody; NOH-1 antibody; NOH1 antibody; NOX-1 antibody; Nox1 antibody; NOX1_HUMAN antibody; RP1 146H21.1 antibody; anti-NOX1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
564
Applicable Applications for anti-NOX1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NOX1 (354-374aa, HIRAAGDWTENLIRAFEQQYS).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-NOX1 antibody, AAA44951, Western blottingAll lanes: Anti NOX1 (AAA44951) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 65KDObserved bind size: 65KD)
WB (Western Blot)
(Anti-NOX1 antibody, AAA44951, Western blottingAll lanes: Anti NOX1 (AAA44951) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 65KDObserved bind size: 65KD)
IHC (Immunohistochemistry)
(Anti-NOX1 antibody, AAA44951, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-NOX1 antibody, AAA44951, IHC(P)IHC(P): Human Lung Cancer Tissue)
Related Product Information for anti-NOX1 antibody
Description: Rabbit IgG polyclonal antibody for NADPH oxidase 1(NOX1) detection. Tested with WB, IHC-P in Human.
Background: NOX1(NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.
Background: NOX1(NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
58,972 Da
NCBI Official Full Name
NADPH oxidase 1
NCBI Official Synonym Full Names
NADPH oxidase 1
NCBI Official Symbol
NOX1
NCBI Official Synonym Symbols
MOX1; NOH1; NOH-1; GP91-2
NCBI Protein Information
NADPH oxidase 1; mitogenic oxidase 1; NADPH oxidase homolog-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
UniProt Protein Name
NADPH oxidase 1
UniProt Gene Name
NOX1
UniProt Synonym Gene Names
MOX1; NOH1; NOX-1; MOX-1
UniProt Entry Name
NOX1_HUMAN
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Product Notes
The NOX1 nox1 (Catalog #AAA44951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOX1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOX1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NOX1 nox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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