FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-NOX2/Gp91phox/CYBB antibody (AAA126008).Overlay histogram showing U937 cells stained with AAA126008 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX2/Gp91phox/CYBB Antibody (AAA126008, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human NOX2/gp91phox/CYBB Polyclonal Antibody | anti-CYBB antibody
Anti-NOX2/gp91phox/CYBB Antibody Picoband
Gene Names
CYBB; CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
NOX2/gp91phox/CYBB, Antibody; Anti-NOX2/gp91phox/CYBB Antibody Picoband; anti-CYBB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-CYBB antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human NOX2/gp91phox/CYBB recombinant protein (Position: F416-H527).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-NOX2/Gp91phox/CYBB antibody (AAA126008).Overlay histogram showing U937 cells stained with AAA126008 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX2/Gp91phox/CYBB Antibody (AAA126008, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-NOX2/Gp91phox/CYBB antibody (AAA126008).Overlay histogram showing U937 cells stained with AAA126008 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX2/Gp91phox/CYBB Antibody (AAA126008, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of NOX2/Gp91phox/CYBB using anti-NOX2/Gp91phox/CYBB antibody (AAA126008).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human Raji whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2/Gp91phox/CYBB antigen affinity purified polyclonal antibody (#AAA126008) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOX2/Gp91phox/CYBB at approximately 65 kDa. The expected band size for NOX2/Gp91phox/CYBB is at 65 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of NOX2/Gp91phox/CYBB using anti-NOX2/Gp91phox/CYBB antibody (AAA126008).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human Raji whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2/Gp91phox/CYBB antigen affinity purified polyclonal antibody (#AAA126008) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOX2/Gp91phox/CYBB at approximately 65 kDa. The expected band size for NOX2/Gp91phox/CYBB is at 65 kDa.)
Related Product Information for anti-CYBB antibody
NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).
Product Categories/Family for anti-CYBB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
65,336 Da
NCBI Official Full Name
Cytochrome b-245 heavy chain
NCBI Official Synonym Full Names
cytochrome b-245, beta polypeptide
NCBI Official Symbol
CYBB
NCBI Official Synonym Symbols
CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
NCBI Protein Information
cytochrome b-245 heavy chain; CGD91-phox; NADPH oxidase 2; p22 phagocyte B-cytochrome; cytochrome b558 subunit beta; neutrophil cytochrome b 91 kDa polypeptide; heme-binding membrane glycoprotein gp91phox; superoxide-generating NADPH oxidase heavy chain s
UniProt Protein Name
Cytochrome b-245 heavy chain
UniProt Gene Name
CYBB
UniProt Synonym Gene Names
NOX2; Cytochrome b558 subunit beta
UniProt Entry Name
CY24B_HUMAN
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Product Notes
The CYBB cybb (Catalog #AAA126008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOX2/gp91phox/CYBB Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOX2/gp91phox/CYBB can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the CYBB cybb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOX2/gp91phox/CYBB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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