IP (Immunoprecipitation)
(Detection of human NPAT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-NPAT antibody AAA212008 used for IP at 3 ug/mg lysate. NPAT was also immunoprecipitated by rabbit anti-NPAT antibody which recognizes a downstream epitope. For blotting immunoprecipitated NPAT, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human NPAT Polyclonal Antibody | anti-NPAT antibody
Rabbit anti-NPAT Antibody, Affinity Purified
Gene Names
NPAT; E14; p220; E14/NPAT
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
NPAT, Antibody; Rabbit anti-NPAT Antibody, Affinity Purified; nuclear protein of the ataxia telangiectasia mutated locus; p220; nuclear protein, co-activator of histone transcription; nuclear protein of the ATM locus; E14/NPAT; E14; protein NPAT; nuclear protein, ataxia-telangiectasia locus; Protein NPAT; nuclear protein, coactivator of histone transcription; anti-NPAT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NPAT antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 700 and 750
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human NPAT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-NPAT antibody AAA212008 used for IP at 3 ug/mg lysate. NPAT was also immunoprecipitated by rabbit anti-NPAT antibody which recognizes a downstream epitope. For blotting immunoprecipitated NPAT, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NPAT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-NPAT antibody AAA212008 used for IP at 3 ug/mg lysate. NPAT was also immunoprecipitated by rabbit anti-NPAT antibody which recognizes a downstream epitope. For blotting immunoprecipitated NPAT, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human NPAT by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPAT (Cat. No. AAA212008 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human NPAT by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPAT (Cat. No. AAA212008 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-NPAT antibody
NPAT (nuclear protein of the ataxia telangiectasia mutated locus) is required for progression through the G1 and S phases of the cell cycle and for S phase entry. It activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. NPAT also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/Q14207].
Product Categories/Family for anti-NPAT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
154,290 Da
NCBI Official Full Name
protein NPAT isoform 2
NCBI Official Synonym Full Names
nuclear protein, coactivator of histone transcription
NCBI Official Symbol
NPAT
NCBI Official Synonym Symbols
E14; p220; E14/NPAT
NCBI Protein Information
protein NPAT
UniProt Protein Name
Protein NPAT
UniProt Gene Name
NPAT
UniProt Synonym Gene Names
CAND3; E14; Nuclear protein of the ATM locus
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Product Notes
The NPAT npat (Catalog #AAA212008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NPAT Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NPAT can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the NPAT npat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPAT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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