WB (Western Blot)
(Western blot analysis of extracts from Mouse brain tissue, using NPHP4 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit NPHP4 Polyclonal Antibody | anti-NPHP4 antibody
NPHP4 Antibody
Gene Names
NPHP4; POC10; SLSN4
Reactivity
Human, Mouse, dogPredicted: Bovine
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
NPHP4, Antibody; NPHP4 Antibody; KIAA0673; Nephrocystin-4; nephronophthisis 4; Nephroretinin; NPHP4; NPHP4_HUMAN; POC10; POC10 centriolar protein homolog; SLSN4; anti-NPHP4 antibody
Host
Rabbit
Reactivity
Human, Mouse, dog
Predicted: Bovine
Predicted: Bovine
Clonality
Polyclonal
Isotype
IgG
Specificity
NPHP4 Antibody detects endogenous levels of NPHP4.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1426
Applicable Applications for anti-NPHP4 antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human NPHP4.
Tissue Specificity
Expressed in kidney, skeletal muscle, heart and liver, and to a lesser extent in brain and lung.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus; Cytoskeleton
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Mouse brain tissue, using NPHP4 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse brain tissue, using NPHP4 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using NPHP4 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using NPHP4 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NPHP4 antibody
Description: Involved in the organization of apical junctions; the function is proposed to implicate a NPHP1-4-8 module (PubMed:19755384, PubMed:21565611). Does not seem to be strictly required for ciliogenesis (PubMed:21565611). Required for building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Seems to recruit INT to basal bodies of motile cilia which subsequently interacts with actin-modifying proteins such as DAAM1 (By similarity). In cooperation with INVS may downregulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating expression and subcellular location of disheveled proteins. Stabilizes protein levels of JADE1 and promotes its translocation to the nucleus leading to cooperative inhibition of canonical Wnt signaling (PubMed:21498478, PubMed:22654112). Acts as negative regulator of the hippo pathway by association with LATS1 and modifying LATS1-dependent phosphorylation and localization of WWTR1/TAZ (PubMed:21555462).
Subunit Structure: Interacts with NPHP1 (PubMed:15661758). Interacts with NPHP1 and RPGRIP1L/NPHP8; NPHP1, NPHP4 and RPGRIP1L are proposed to form a functional NPHP1-4-8 module localized to cell-cell contacts and the ciliary transition zone; NPHP4 mediates the interaction between NPHP1 and RPGRIP1L. Interacts with IQCB1/NPHP5; the interaction likely requires additional interactors (By similarity). Interacts with RPGRIP1, CEP164, JADE1, MPP5, INADL, PARD6A, INVS, DVL2, LATS1. Interacts with INTU; INTU mediates the interaction between NPHP4 and DAAM1.
Similarity: Belongs to the NPHP4 family.
Subunit Structure: Interacts with NPHP1 (PubMed:15661758). Interacts with NPHP1 and RPGRIP1L/NPHP8; NPHP1, NPHP4 and RPGRIP1L are proposed to form a functional NPHP1-4-8 module localized to cell-cell contacts and the ciliary transition zone; NPHP4 mediates the interaction between NPHP1 and RPGRIP1L. Interacts with IQCB1/NPHP5; the interaction likely requires additional interactors (By similarity). Interacts with RPGRIP1, CEP164, JADE1, MPP5, INADL, PARD6A, INVS, DVL2, LATS1. Interacts with INTU; INTU mediates the interaction between NPHP4 and DAAM1.
Similarity: Belongs to the NPHP4 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 150 kDa
Predicted: 158 kDa
Predicted: 158 kDa
NCBI Official Full Name
nephrocystin-4 isoform a
NCBI Official Synonym Full Names
nephrocystin 4
NCBI Official Symbol
NPHP4
NCBI Official Synonym Symbols
POC10; SLSN4
NCBI Protein Information
nephrocystin-4
UniProt Protein Name
Nephrocystin-4
UniProt Gene Name
NPHP4
UniProt Synonym Gene Names
KIAA0673
UniProt Entry Name
NPHP4_HUMAN
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Product Notes
The NPHP4 nphp4 (Catalog #AAA328693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPHP4 Antibody reacts with Human, Mouse, dog Predicted: Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's NPHP4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the NPHP4 nphp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPHP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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