WB (Western Blot)
(Anti-NQO1 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:31 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human NQO1 Polyclonal Antibody | anti-NQO1 antibody
Anti-NQO1 Antibody, Rabbit Polyclonal
Gene Names
NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
NQO1, Antibody; Anti-NQO1 Antibody, Rabbit Polyclonal; NQO1 Antibody, Rabbit PAb, Antigen Affinity Purified; NAD(P)H dehydrogenase, quinone 1; Anti-DHQU Antibody; Anti-DIA4 Antibody; Anti-DTD Antibody; Anti-NMOR1 Antibody; Anti-NMORI Antibody; Anti-QR1 Antibody; anti-NQO1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NQO1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NQO1 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human NQO1 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human NQO1 (rh NQO1). NQO1 specific IgG was purified by Human NQO1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-NQO1 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:31 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-NQO1 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:31 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(NQO1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-NQO1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NQO1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 31 kDa)
IP (Immunoprecipitation)
(NQO1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-NQO1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NQO1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 31 kDa)
Related Product Information for anti-NQO1 antibody
NQO1 gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. NQO1 forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one-electron reduction of quinones that results in the production of radical species. Mutations in the NQO1 gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Recent pharmacological research suggests the feasibility of genotype-directed redox chemotherapeutic intervention targeting NQO1 breast cancer, a common missense genotype encoding a functionally impaired NQO1 protein.
References
Ross David. et al., 2002, J Biol Chem. 277 (16): 14060-7. Cabello CM. et al., 2010, Free Radic Res. 45 (3): 276-92. Adesnik M. et al., 1988, J Biol Chem. 263 (27): 13572-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
274
NCBI Official Full Name
NAD(P)H dehydrogenase
NCBI Official Synonym Full Names
NAD(P)H dehydrogenase, quinone 1
NCBI Official Symbol
NQO1
NCBI Official Synonym Symbols
DTD; QR1; DHQU; DIA4; NMOR1; NMORI
NCBI Protein Information
NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; azoreductase; diaphorase-4; DT-diaphorase; dioxin-inducible 1; menadione reductase; quinone reductase 1; phylloquinone reductase; NAD(P)H:quinone oxireductase; NAD(P)H:quinone oxidoredu
UniProt Protein Name
NAD(P)H dehydrogenase [quinone] 1
UniProt Gene Name
NQO1
UniProt Synonym Gene Names
DIA4; NMOR1; DTD; QR1
UniProt Entry Name
NQO1_HUMAN
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Product Notes
The NQO1 nqo1 (Catalog #AAA255510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NQO1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NQO1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NQO1 nqo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NQO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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