FCM/FACS (Flow Cytometry)
(NR1H3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human NR1H3 Polyclonal Antibody | anti-NR1H3 antibody
NR1H3 Antibody (Center)
Gene Names
NR1H3; LXRA; LXR-a; RLD-1
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NR1H3, Antibody; NR1H3 Antibody (Center); Oxysterols receptor LXR-alpha; Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3; NR1H3; LXRA; anti-NR1H3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NR1H3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-253 amino acids from the Central region of human NR1H3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
226-253
Sequence Length
387
Applicable Applications for anti-NR1H3 antibody
ELISA, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(NR1H3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(NR1H3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of NR1H3 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). NR1H3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of NR1H3 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). NR1H3 (arrow) was detected using the purified Pab.)
IF (Immunofluorescence)
(Fluorescent image of U251 cell stained with NR1H3 Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR1H3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NR1H3 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent image of U251 cell stained with NR1H3 Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR1H3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NR1H3 immunoreactivity is localized to Nucleus significantly.)
Related Product Information for anti-NR1H3 antibody
NR1H3 form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements.
Product Categories/Family for anti-NR1H3 antibody
References
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Nedumaran, B., et al. J. Biol. Chem. 285(12):9221-9232(2010)
Zhao, C., et al. J. Endocrinol. 204(3):233-240(2010)
Anthonisen, E.H., et al. J. Biol. Chem. 285(3):1607-1615(2010)
Nedumaran, B., et al. J. Biol. Chem. 285(12):9221-9232(2010)
Zhao, C., et al. J. Endocrinol. 204(3):233-240(2010)
Anthonisen, E.H., et al. J. Biol. Chem. 285(3):1607-1615(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50396
NCBI Official Full Name
oxysterols receptor LXR-alpha isoform 2
NCBI Official Synonym Full Names
nuclear receptor subfamily 1, group H, member 3
NCBI Official Symbol
NR1H3
NCBI Official Synonym Symbols
LXRA; LXR-a; RLD-1
NCBI Protein Information
oxysterols receptor LXR-alpha
UniProt Protein Name
Oxysterols receptor LXR-alpha
UniProt Gene Name
NR1H3
UniProt Synonym Gene Names
LXRA
UniProt Entry Name
NR1H3_HUMAN
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Product Notes
The NR1H3 nr1h3 (Catalog #AAA288850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 226-253. The NR1H3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NR1H3 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NR1H3 nr1h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR1H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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