IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NR2C2 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly.)
Rabbit NR2C2 Polyclonal Antibody | anti-NR2C2 antibody
NR2C2 Antibody (C-term)
Gene Names
NR2C2; TR4; TAK1
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NR2C2, Antibody; NR2C2 Antibody (C-term); Nuclear receptor subfamily 2 group C member 2; Orphan nuclear receptor TAK1; Orphan nuclear receptor TR4; Testicular receptor 4; NR2C2; TAK1; TR4; anti-NR2C2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NR2C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-466 amino acids from the C-terminal region of human NR2C2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
438-466
Sequence Length
615
Applicable Applications for anti-NR2C2 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NR2C2 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NR2C2 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly.)
WB (Western Blot)
(NR2C2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NR2C2 antibody detected the NR2C2 protein (arrow).)
WB (Western Blot)
(NR2C2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NR2C2 antibody detected the NR2C2 protein (arrow).)
Related Product Information for anti-NR2C2 antibody
Members of the nuclear hormone receptor family, such as
NR2C2, act as ligand-activated transcription factors. The proteins
have an N-terminal transactivation domain, a central DNA-binding
domain with 2 zinc fingers, and a ligand-binding domain at the C
terminus. The activated receptor/ligand complex is translocated to
the nucleus where it binds to hormone response elements of target
genes (Yoshikawa et al., 1996 [PubMed 8661150]).
NR2C2, act as ligand-activated transcription factors. The proteins
have an N-terminal transactivation domain, a central DNA-binding
domain with 2 zinc fingers, and a ligand-binding domain at the C
terminus. The activated receptor/ligand complex is translocated to
the nucleus where it binds to hormone response elements of target
genes (Yoshikawa et al., 1996 [PubMed 8661150]).
Product Categories/Family for anti-NR2C2 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Srivastava, R., et al. J. Biol. Chem. 285(15):11100-11105(2010)
Ear, T., et al. J. Immunol. 184(7):3897-3906(2010)
Suzuki, S., et al. J. Biol. Chem. 285(7):4441-4446(2010)
Huang, Y.H., et al. J. Cell. Physiol. 222(2):347-356(2010)
Srivastava, R., et al. J. Biol. Chem. 285(15):11100-11105(2010)
Ear, T., et al. J. Immunol. 184(7):3897-3906(2010)
Suzuki, S., et al. J. Biol. Chem. 285(7):4441-4446(2010)
Huang, Y.H., et al. J. Cell. Physiol. 222(2):347-356(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65414
NCBI Official Full Name
nuclear receptor subfamily 2 group C member 2 isoform 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 2, group C, member 2
NCBI Official Symbol
NR2C2
NCBI Official Synonym Symbols
TR4; TAK1
NCBI Protein Information
nuclear receptor subfamily 2 group C member 2
UniProt Protein Name
Nuclear receptor subfamily 2 group C member 2
UniProt Gene Name
NR2C2
UniProt Synonym Gene Names
TAK1; TR4
UniProt Entry Name
NR2C2_HUMAN
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Product Notes
The NR2C2 nr2c2 (Catalog #AAA287410) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 438-466. The NR2C2 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NR2C2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NR2C2 nr2c2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR2C2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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