IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NR3C1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR3C1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Rabbit NR3C1 Polyclonal Antibody | anti-NR3C1 antibody
NR3C1 Antibody (Center)
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, mouse (Predicted Reactivity: Rabbit)
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NR3C1, Antibody; NR3C1 Antibody (Center); Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1; NR3C1; GRL; anti-NR3C1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rabbit)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NR3C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-262 amino acids from the Central region of human NR3C1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
236-262
Sequence Length
751
Applicable Applications for anti-NR3C1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NR3C1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR3C1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NR3C1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR3C1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
IHC (Immunohiostchemistry)
(NR3C1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR3C1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(NR3C1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR3C1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(NR3C1 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected NR3C1 protein (arrow).)
WB (Western Blot)
(NR3C1 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected NR3C1 protein (arrow).)
Related Product Information for anti-NR3C1 antibody
NR3C1 is a receptor for
glucocorticoids that can act as both a transcription factor and as
a regulator of other transcription factors. This protein can also
be found in heteromeric cytoplasmic complexes along with heat shock
factors and immunophilins. The protein is typically found in the
cytoplasm until it binds a ligand, which induces transport into the
nucleus. Mutations in this gene are a cause of glucocorticoid
resistance, or cortisol, resistance. Alternate splicing, the use of
at least three different promoters, and alternate translation
initiation sites result in several transcript variants encoding the
same protein or different isoforms, but the full-length nature of
some variants has not been determined.
glucocorticoids that can act as both a transcription factor and as
a regulator of other transcription factors. This protein can also
be found in heteromeric cytoplasmic complexes along with heat shock
factors and immunophilins. The protein is typically found in the
cytoplasm until it binds a ligand, which induces transport into the
nucleus. Mutations in this gene are a cause of glucocorticoid
resistance, or cortisol, resistance. Alternate splicing, the use of
at least three different promoters, and alternate translation
initiation sites result in several transcript variants encoding the
same protein or different isoforms, but the full-length nature of
some variants has not been determined.
Product Categories/Family for anti-NR3C1 antibody
References
Bosker, F.J., et al. Mol. Psychiatry (2010)
Inkster, B., et al. Neuroimage (2010)
Geelhoed, M.J., et al. BMC Med. Genet. 11, 39 (2010)
Inkster, B., et al. Neuroimage (2010)
Geelhoed, M.J., et al. BMC Med. Genet. 11, 39 (2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85659
NCBI Official Full Name
glucocorticoid receptor isoform alpha
NCBI Official Synonym Full Names
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
NCBI Official Symbol
NR3C1
NCBI Official Synonym Symbols
GR; GCR; GRL; GCCR; GCRST
NCBI Protein Information
glucocorticoid receptor
UniProt Protein Name
Glucocorticoid receptor
UniProt Gene Name
NR3C1
UniProt Synonym Gene Names
GRL; GR
UniProt Entry Name
GCR_HUMAN
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Product Notes
The NR3C1 nr3c1 (Catalog #AAA286638) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 236-262. The NR3C1 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's NR3C1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NR3C1 nr3c1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR3C1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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