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IHC (Immunohistochemistry)
(Anti- NR3C1 Picoband antibody, AAA46374, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue)
NR3C1 Polyclonal Antibody | anti-NR3C1 antibody
Anti-NR3C1 Antibody
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
NR3C1, Antibody; Anti-NR3C1 Antibody; Glucocorticoid receptor; GCCR; GCR; GCR_HUMAN; GCRST; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor lymphocyte; GR; GRL; Grl1; NR3C1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); anti-NR3C1 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
777
Applicable Applications for anti-NR3C1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human NR3C1 recombinant protein (Position: A20-F199). Human NR3C1 shares 80% and 74% amino acid (aa) sequence identity with mouse and rat NR3C1, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti- NR3C1 Picoband antibody, AAA46374, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue)
IHC (Immunohistochemistry)
(Anti- NR3C1 Picoband antibody, AAA46374, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue)
IHC (Immunohistochemisry)
(Anti- NR3C1 Picoband antibody, AAA46374, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- NR3C1 Picoband antibody, AAA46374, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti- NR3C1 Picoband antibody, AAA46374, IHC(P)IHC(P): Rat Cardiac Muscle Tissue)
IHC (Immunohiostchemistry)
(Anti- NR3C1 Picoband antibody, AAA46374, IHC(P)IHC(P): Rat Cardiac Muscle Tissue)
WB (Western Blot)
(Anti- NR3C1 Picoband antibody, AAA46374, Western blottingAll lanes: Anti NR3C1 (AAA46374) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Rat Spleen Tissue Lysate at 50ugLane 4: Human Placenta Tissue Lysate at 50ugLane 5: SMMC Whole Cell Lysate at 40ugLane 6: HEPA Whole Cell Lysate at 40ugLane 7: NEURO Whole Cell Lysate at 40ugLane 8: HELA Whole Cell Lysate at 40ugLane 9: PC-12 Whole Cell Lysate at 40ugPredicted bind size: 86KDObserved bind size: 100KD)
WB (Western Blot)
(Anti- NR3C1 Picoband antibody, AAA46374, Western blottingAll lanes: Anti NR3C1 (AAA46374) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Rat Spleen Tissue Lysate at 50ugLane 4: Human Placenta Tissue Lysate at 50ugLane 5: SMMC Whole Cell Lysate at 40ugLane 6: HEPA Whole Cell Lysate at 40ugLane 7: NEURO Whole Cell Lysate at 40ugLane 8: HELA Whole Cell Lysate at 40ugLane 9: PC-12 Whole Cell Lysate at 40ugPredicted bind size: 86KDObserved bind size: 100KD)
Related Product Information for anti-NR3C1 antibody
Description: Rabbit IgG polyclonal antibody for Glucocorticoid receptor(NR3C1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31). GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus).
Background: The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31). GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus).
References
1. Lu NZ, Wardell SE, Burnstein KL, Defranco D, Fuller PJ, Giguere V, Hochberg RB, McKay L, Renoir JM, Weigel NL, Wilson EM, McDonnell DP, Cidlowski JA (2006). "International Union of Pharmacology. LXV. The pharmacology and classification of the nuclear receptor superfamily: glucocorticoid, mineralocorticoid, progesterone, and androgen receptors". Pharmacol Revl 58 (4): 782-97. 2. Rhen T, Cidlowski JA (October 2005). "Antiinflammatory action of glucocorticoids--new mechanisms for old drugs". N. Engl. J. Med. 353 (16): 1711-23. 3. Hollenberg SM, Weinberger C, Ong ES, Cerelli G, Oro A, Lebo R, Thompson EB, Rosenfeld MG, Evans RM (1985). "Primary structure and expression of a functional human glucocorticoid receptor cDNA". Nature 318 (6047): 635-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,802 Da
NCBI Official Full Name
glucocorticoid receptor isoform alpha
NCBI Official Synonym Full Names
nuclear receptor subfamily 3 group C member 1
NCBI Official Symbol
NR3C1
NCBI Official Synonym Symbols
GR; GCR; GRL; GCCR; GCRST
NCBI Protein Information
glucocorticoid receptor
UniProt Protein Name
Glucocorticoid receptor
UniProt Gene Name
NR3C1
UniProt Synonym Gene Names
GRL; GR
UniProt Entry Name
GCR_HUMAN
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Product Notes
The NR3C1 nr3c1 (Catalog #AAA46374) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-NR3C1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NR3C1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NR3C1 nr3c1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR3C1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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