Rabbit anti-Human NR3C1 Polyclonal Antibody | anti-NR3C1 antibody

Anti-NR3C1 Antibody, Rabbit Polyclonal

Cat. Num. AAA257504

• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: NR3C1, Antibody; Anti-NR3C1 Antibody, Rabbit Polyclonal; NR3C1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-GCCR Antibody; Anti-GCR Antibody; Anti-GCRST Antibody; Anti-GR Antibody; Anti-GRL Antibody; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); anti-NR3C1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NR3C1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-NR3C1 antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
• Immunogen: A synthetic peptide corresponding to the center region of the Human NR3C1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human NR3C1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

IP (Immunoprecipitation)

(NR3C1 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-NR3C1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NR3C1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 86 kDaObserved band size: 86 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of NR3C1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NR3C1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)

IHC (Immunohistochemistry)

(Immunochemical staining of human NR3C1 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-NR3C1 antibody

Background: NR3C1 (Nuclear Receptor Subfamily 3 Group C Member 1) is a Protein Coding gene. This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. NR3C1 is a transcriptional regulator of many drug-metabolizing enzymes and anti-inflammatory molecules. NR3C1 polymorphisms associate with obesity, muscle strength, and cortisol sensitivity. Glucocorticoid receptor gene polymorphism (NR3C1 646 C>G) may play an important role in the development of severe bronchial asthma and resistance to glucocorticoids (GCs). Disturbances in the structure and function of the glucocorticoid receptor (GR) alter the glucocorticoid regulation of the corticotropin-releasing hormone, which leads to nonspecific activation of numerous receptors in the brain and alters the metabolism.