WB (Western Blot)
(Detection of human NRIP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NRIP antibody AAA211874 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NRIP was also immunoprecipitated by rabbit anti-NRIP antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
Rabbit anti-Human NRIP Polyclonal Antibody | anti-NRIP antibody
Rabbit anti-NRIP Antibody, Affinity Purified
Gene Names
DCAF6; NRIP; ARCAP; IQWD1; PC326; MSTP055; 1200006M05Rik
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NRIP, Antibody; Rabbit anti-NRIP Antibody, Affinity Purified; IQ motif and WD repeat-containing protein 1; nuclear receptor interaction protein; NRIP; MSTP055; IQ motif and WD repeats 1; PC326; DDB1- and CUL4-associated factor 6; ARCAP; androgen receptor complex-associated protein; 1200006M05Rik; IQWD1; DDB1 and CUL4 associated factor 6; anti-NRIP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-NRIP antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 600 and 650
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NRIP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NRIP antibody AAA211874 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NRIP was also immunoprecipitated by rabbit anti-NRIP antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human NRIP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NRIP antibody AAA211874 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NRIP was also immunoprecipitated by rabbit anti-NRIP antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human NRIP by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NRIP (Cat. No. AAA211874 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human NRIP by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NRIP (Cat. No. AAA211874 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-NRIP antibody
Nuclear receptor interaction protein (NRIP) was isolated as a ligand-dependent coactivator of the androgen receptor (AR) and glucocorticoid receptor (GR). NRIP was found to enhance the promoter activity of the AR-driven PSA promoter. NRIP is a target gene for AR and Sp1, and complex formation between AR and NRIP protects AR protein from proteasome degradation.
Product Categories/Family for anti-NRIP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103,070 Da
NCBI Official Full Name
DDB1- and CUL4-associated factor 6 isoform a
NCBI Official Synonym Full Names
DDB1 and CUL4 associated factor 6
NCBI Official Symbol
DCAF6
NCBI Official Synonym Symbols
NRIP; ARCAP; IQWD1; PC326; MSTP055; 1200006M05Rik
NCBI Protein Information
DDB1- and CUL4-associated factor 6; IQ motif and WD repeats 1; nuclear receptor interaction protein; IQ motif and WD repeat-containing protein 1; androgen receptor complex-associated protein
UniProt Protein Name
DDB1- and CUL4-associated factor 6
UniProt Gene Name
DCAF6
UniProt Synonym Gene Names
IQWD1; ARCAP; NRIP
UniProt Entry Name
DCAF6_HUMAN
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Product Notes
The NRIP dcaf6 (Catalog #AAA211874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NRIP Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NRIP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NRIP dcaf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NRIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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