Rabbit anti-Human, rat NSE (Neuron specific enolase) Polyclonal Antibody | anti-ENO2 antibody

Anti-Neuron Specific Enolase (NSE)

Cat. Num. AAA72800

• Gene Names: ENO2; NSE; HEL-S-279
• Reactivity: Human, rat
• Applications: Immunofluorescence, Western Blot
• Purity: Neat Serum (Unpurified neat serum.)
• Synonyms: NSE (Neuron specific enolase), Antibody; Anti-Neuron Specific Enolase (NSE); anti-ENO2 antibody

• Host: Rabbit
• Reactivity: Human, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~47kDa NSE protein.
• Purity/Purification: Neat Serum (Unpurified neat serum.)
• Form/Format: Liquid.
• Sequence Length: 434

• Applicable Applications for anti-ENO2 antibody: IF (Immunofluorescence), WB (Western Blot)
• Antigen: Recombinant human NSE expressed in and purified from E. coli.
• Immunogen Information: Recombinant human NSE expressed in and purified from E. coli
• Immunogen Species: Human
• Species Reactivity Note: The antibody has been directly tested for reactivity in human and rat. It is expected that it will work on other mammalian tissues.
• Biological Significance: Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Application Data

Related Product Information for anti-ENO2 antibody

Rabbit polyclonal antibody

References

• MacAlesse SM, Dunbar B, Fotherqill JE, Hinks LJ, Day IN (1988) Complete amino acid sequence of neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE). Eur J Biochem. 178(2):413-17.
• Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. J. Neurotrauma 23:1201-1210 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5803011
• NCBI GeneID: 2026
• NCBI Accession #: NP_001966.1
• NCBI GenBank Nucleotide #: NM_001975.2
• UniProt Accession #: P09104; Q96J33
• Molecular Weight: 47
• NCBI Official Full Name: gamma-enolase
• NCBI Official Synonym Full Names: enolase 2 (gamma, neuronal)
• NCBI Official Symbol: ENO2
• NCBI Official Synonym Symbols: NSE; HEL-S-279
• NCBI Protein Information: gamma-enolase; neural enolase; neuron-specific enolase; neurone-specific enolase; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; epididymis secretory protein Li 279
• UniProt Protein Name: Gamma-enolase
• UniProt Gene Name: ENO2
• UniProt Synonym Gene Names: NSE
• UniProt Entry Name: ENOG_HUMAN