Rabbit anti-Human NSFL1C Polyclonal Antibody | anti-NSFL1C antibody

Anti-NSFL1C Antibody, Rabbit Polyclonal

Cat. Num. AAA255953

• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NSFL1C, Antibody; Anti-NSFL1C Antibody, Rabbit Polyclonal; NSFL1C Antibody, Rabbit PAb, Antigen Affinity Purified; NSFL1 (p97) cofactor (p47); Anti-dJ776F14.1 Antibody; Anti-P47 Antibody; Anti-UBX1 Antibody; Anti-UBXD10 Antibody; Anti-UBXN2C Antibody; anti-NSFL1C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NSFL1C
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NSFL1C antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human NSFL1C fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human NSFL1C fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(NSFL1C was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-NSFL1C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NSFL1C rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size :46 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of NSFL1C in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NSFL1C polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus / Cytoplasm and Cell membrane.)

WB (Western Blot)

(Anti-NSFL1C rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: A431 Whole Cell LysateLane C: H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:41 kDa)

Related Product Information for anti-NSFL1C antibody

NSFL1C (NSFL1 Cofactor) is a Protein Coding gene. The NSFL1C gene, located on 20p13, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and rice. N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. The related pathways of NSFL1C are Protein processing in the endoplasmic reticulum.

References

Zhang X, et al. (2015) Altered cofactor regulation with disease-associated p97/vcp mutations. Proc Natl Acad Sci U S A 112 (14): E1705-1714.Park ES, et al. (2017) The opposite role of two uba-ubx containing proteins, p47 and saks1 in the degradation of a single erad substrate, alpha-tcr. Mol Cell Biochem 425 (1-2): 37-45.Kondo H, et al. (1997) P47 is a cofactor for p97-mediated membrane fusion. Nature 388 (6637): 75-78.