IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using NT5E antibody. Blue: DAPI for nuclear staining.)
Rabbit NT5E Polyclonal Antibody | anti-NT5E antibody
NT5E Antibody
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Synonyms
NT5E, Antibody; NT5E Antibody; CD73; E5NT; NT; NT5; NTE; eN; Ent; anti-NT5E antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
1mg/mL (varies by lot)
Sequence Length
264
Applicable Applications for anti-NT5E antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant protein of human NT5E
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-14682 / sc-14684 / sc-25603
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using NT5E antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using NT5E antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using NT5E antibody at dilution of 1:100 (400x lens).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using NT5E antibody at dilution of 1:100 (400x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using NT5E antibody)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using NT5E antibody)
Related Product Information for anti-NT5E antibody
Ecto-5'nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleoside (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine-monophosphate, therefore driving the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promotor leads to upregulation of NT5E during hypoxia (3). NT5E biological roles span from lymphocyte adhesion (4, 5), to fibrosis (6), to regulation of nociception (7, 8).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,949 Da
NCBI Official Full Name
NT5E protein
NCBI Official Synonym Full Names
5'-nucleotidase, ecto (CD73)
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
NCBI Protein Information
5'-nucleotidase; 5'-NT; Purine 5-Prime-Nucleotidase; ecto-5'-nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
UniProt Entry Name
5NTD_HUMAN
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Product Notes
The NT5E nt5e (Catalog #AAA169026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NT5E Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NT5E can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NT5E nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NT5E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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