WB (Western Blot)
(Western blot analysis of extracts from various samples, using MAP4 Antibody.Lane 1: PC12(H2O2 treatment), blocked with antigen-specific peptides,Lane 2: PC12(H2O2 treatment),Lane 3: Hela cells(heat shock treatment),Lane 4: HepG2 cells(serum starvation treatment).Observed bands: 200kDa)
Rabbit Nuclear factor 1 C-type Polyclonal Antibody | anti-NFIC antibody
Nuclear factor 1 C-type Antibody
Gene Names
NFIC; CTF; NFI; CTF5; NF-I
Reactivity
Human, Mouse, Rat, Monkey
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Nuclear factor 1 C-type, Antibody; Nuclear factor 1 C-type Antibody; Nuclear factor 1 C-type; NFIC; NF1-C; Nuclear factor 1/C; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/C; NF-I/C; NFI-C; TGGCA-binding protein; NFI; anti-NFIC antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Nuclear Factor 1 C-type Antibody detects endogenous levels of total Nuclear Factor 1 C-type.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NFIC antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human Nuclear Factor 1 C-type.
Conjugation
Unconjugated
Similarity
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. Belongs to the CTF/NF-I family.
Subunit Structure
Binds DNA as a homodimer.
Function
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from various samples, using MAP4 Antibody.Lane 1: PC12(H2O2 treatment), blocked with antigen-specific peptides,Lane 2: PC12(H2O2 treatment),Lane 3: Hela cells(heat shock treatment),Lane 4: HepG2 cells(serum starvation treatment).Observed bands: 200kDa)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using MAP4 Antibody.Lane 1: PC12(H2O2 treatment), blocked with antigen-specific peptides,Lane 2: PC12(H2O2 treatment),Lane 3: Hela cells(heat shock treatment),Lane 4: HepG2 cells(serum starvation treatment).Observed bands: 200kDa)
IHC (Immunohiostchemistry)
(At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Nuclear factor 1 C-type Antibody.Lane 1: HepG2 cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: HepG2 cells(heat shock treatment),Lane 3: P19 cells(serum starvation treatment),Lane 4: VERO cells(H2O2 treatment).)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Nuclear factor 1 C-type Antibody.Lane 1: HepG2 cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: HepG2 cells(heat shock treatment),Lane 3: P19 cells(serum starvation treatment),Lane 4: VERO cells(H2O2 treatment).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56
NCBI Official Full Name
nuclear factor 1 C-type isoform 1
NCBI Official Synonym Full Names
nuclear factor I C
NCBI Official Symbol
NFIC
NCBI Official Synonym Symbols
CTF; NFI; CTF5; NF-I
NCBI Protein Information
nuclear factor 1 C-type
UniProt Protein Name
Nuclear factor 1 C-type
UniProt Gene Name
NFIC
UniProt Synonym Gene Names
NFI; NF1-C; Nuclear factor 1/C; CTF; NF-I/C; NFI-C
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Product Notes
The NFIC nfic (Catalog #AAA330031) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nuclear factor 1 C-type Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclear factor 1 C-type can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NFIC nfic for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuclear factor 1 C-type, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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