WB (Western Blot)
(Detection of human and mouse NUDCD3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUDCD3 antibody AAA212983 (lot AAA212983-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human NUDCD3 Polyclonal Antibody | anti-NUDCD3 antibody
Rabbit anti-NUDCD3 Antibody, Affinity Purified
Gene Names
NUDCD3; NudCL
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NUDCD3, Antibody; Rabbit anti-NUDCD3 Antibody, Affinity Purified; nudC domain-containing protein 3; NudCL; NudC-like protein; NudC domain-containing protein 3; NudC domain containing 3; anti-NUDCD3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NUDCD3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Orangutan
Immunogen
Between 311 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NUDCD3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUDCD3 antibody AAA212983 (lot AAA212983-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse NUDCD3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUDCD3 antibody AAA212983 (lot AAA212983-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human NUDCD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUDCD3 antibody AAA212983 (lot AAA212983-1) used for IP at 6 ug per reaction. NUDCD3 was also immunoprecipitated by rabbit anti-NUDCD3 antibody For blotting immunoprecipitated NUDCD3, AAA212983 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NUDCD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUDCD3 antibody AAA212983 (lot AAA212983-1) used for IP at 6 ug per reaction. NUDCD3 was also immunoprecipitated by rabbit anti-NUDCD3 antibody For blotting immunoprecipitated NUDCD3, AAA212983 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-NUDCD3 antibody
NudC domain-containing protein 3 (NUDCD3) functions to maintain the stability of dynein intermediate chain. Depletion NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. The protein localizes to the Golgi apparatus during interphase, and levels of the protein increase after the G1/S transition [taken from NCBI Entrez Gene (Gene ID: 23386)].
Product Categories/Family for anti-NUDCD3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,822 Da
NCBI Official Full Name
nudC domain-containing protein 3
NCBI Official Synonym Full Names
NudC domain containing 3
NCBI Official Symbol
NUDCD3
NCBI Official Synonym Symbols
NudCL
NCBI Protein Information
nudC domain-containing protein 3; NudC-like protein
UniProt Protein Name
NudC domain-containing protein 3
UniProt Gene Name
NUDCD3
UniProt Synonym Gene Names
KIAA1068
UniProt Entry Name
NUDC3_HUMAN
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Product Notes
The NUDCD3 nudcd3 (Catalog #AAA212983) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NUDCD3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUDCD3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NUDCD3 nudcd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUDCD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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