IP (Immunoprecipitation)
(Nuf2 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate1 uL anti-Nuf2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Nuf2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 51 kDaObserved band size: 51 kDa)
Rabbit anti-Human Nuf2 Polyclonal Antibody | anti-NUF2 antibody
Anti-Nuf2 Antibody, Rabbit Polyclonal
Gene Names
NUF2; CDCA1; CT106; NUF2R
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Nuf2, Antibody; Anti-Nuf2 Antibody, Rabbit Polyclonal; NUF2 Antibody, Rabbit PAb, Antigen Affinity Purified; NUF2, NDC80 kinetochore complex component; Anti-CDCA1 Antibody; Anti-CT106 Antibody; Anti-NUF2R Antibody; anti-NUF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NUF2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NUF2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human NUF2
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human NUF2, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(Nuf2 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate1 uL anti-Nuf2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Nuf2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 51 kDaObserved band size: 51 kDa)
IP (Immunoprecipitation)
(Nuf2 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate1 uL anti-Nuf2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Nuf2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 51 kDaObserved band size: 51 kDa)
WB (Western Blot)
(Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-NUF2 antibody
NUF2 (NUF2 Component Of NDC80 Kinetochore Complex) is a Protein Coding gene. This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. It belongs to the NUF2 family. NUF2, which is essential for kinetochore-microtubule attachment in mitosis, has emerged as a critical mediator of the cell cycle in multiple tumor occurrences. It has been reported that NUF2 is associated with multiple human cancers. NUF2 is consistently overexpressed in human PC and NUF2 is closely linked with human PC progression through the meditator LncRNA AF339813. As an important component of the NDC80 kinetochore complex, NUF2 is essential for kinetochore-microtubule attachment and chromosome segregation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
464
NCBI Official Full Name
Kinetochore protein Nuf2
NCBI Official Synonym Full Names
NUF2, NDC80 kinetochore complex component
NCBI Official Symbol
NUF2
NCBI Official Synonym Symbols
CDCA1; CT106; NUF2R
NCBI Protein Information
kinetochore protein Nuf2; hNuf2; hNuf2R; hsNuf2; cancer/testis antigen 106; cell division cycle associated 1; cell division cycle-associated protein 1; NUF2, NDC80 kinetochore complex component, homolog
UniProt Protein Name
Kinetochore protein Nuf2
UniProt Gene Name
NUF2
UniProt Synonym Gene Names
CDCA1; NUF2R; hNuf2; hNuf2R; hsNuf2
UniProt Entry Name
NUF2_HUMAN
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Product Notes
The NUF2 nuf2 (Catalog #AAA254718) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nuf2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nuf2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NUF2 nuf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuf2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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