WB (Western Blot)
(Detection of human and mouse NUP35 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody AAA211629 (lot AAA211629-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse NUP35 Polyclonal Antibody | anti-NUP35 antibody
Rabbit anti-NUP35 Antibody, Affinity Purified
Gene Names
NUP35; MP44; NP44; MP-44; NUP53
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NUP35, Antibody; Rabbit anti-NUP35 Antibody, Affinity Purified; NP44; NUP53; nucleoporin NUP53; nucleoporin NUP35; nuclear pore complex protein Nup53; MP-44; MP44; mitotic phosphoprotein 44; 35 kDa nucleoporin; nucleoporin 35kDa; Nucleoporin NUP53; nucleoporin 35; anti-NUP35 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NUP35 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 276 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NUP35 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody AAA211629 (lot AAA211629-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse NUP35 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody AAA211629 (lot AAA211629-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NUP35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP35 antibody AAA211629 (lot AAA211629-2) used for IP at 6 ug per reaction. NUP35 was also immunoprecipitated by a previous lot of this antibody (lot AAA211629-1) and rabbit anti-NUP35 antibody BL6800 For blotting immunoprecipitated NUP35, AAA211629 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human NUP35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP35 antibody AAA211629 (lot AAA211629-2) used for IP at 6 ug per reaction. NUP35 was also immunoprecipitated by a previous lot of this antibody (lot AAA211629-1) and rabbit anti-NUP35 antibody BL6800 For blotting immunoprecipitated NUP35, AAA211629 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human NUP35 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NUP35 (Cat. No. AAA211629 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human NUP35 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NUP35 (Cat. No. AAA211629 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-NUP35 antibody
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. There are about 30 different human Nups that have been identified. Human Nup35 is the 35 kDa ortholog of the yeast Nup53p. Nup35 is also referred to as Nup53 or vertebrate Nup53. Nup35 is a component of a NPC subcomplex that contains Nup35, Nup93, Nup155, and Nup205.
Product Categories/Family for anti-NUP35 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,142 Da
NCBI Official Full Name
nucleoporin NUP53 isoform a
NCBI Official Synonym Full Names
nucleoporin 35kDa
NCBI Official Symbol
NUP35
NCBI Official Synonym Symbols
MP44; NP44; MP-44; NUP53
NCBI Protein Information
nucleoporin NUP53; mitotic phosphoprotein 44; nuclear pore complex protein Nup53
UniProt Protein Name
Nucleoporin NUP53
UniProt Gene Name
NUP35
UniProt Synonym Gene Names
MP44; NUP53; MP-44
UniProt Entry Name
NUP53_HUMAN
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Product Notes
The NUP35 nup35 (Catalog #AAA211629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NUP35 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NUP35 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NUP35 nup35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUP35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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