Rabbit anti-Human NUP62 Polyclonal Antibody | anti-NUP62 antibody

Anti-NUP62 Antibody, Rabbit Polyclonal

Cat. Num. AAA256622

• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NUP62, Antibody; Anti-NUP62 Antibody, Rabbit Polyclonal; NUP62 Antibody, Rabbit PAb, Antigen Affinity Purified; nucleoporin 62kDa; Anti-IBSN Antibody; Anti-p62 Antibody; Anti-SNDI Antibody; anti-NUP62 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NUP62
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NUP62 antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human NUP62 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human NUP62 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(NUP62 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-NUP62 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NUP62 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 53 kDaObserved band size :70 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of NUP62 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NUP62 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nuclear membrane.)

WB (Western Blot)

(Anti-NUP62 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: MOLT-4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:53 kDaObserved band size:70 kDa)

Related Product Information for anti-NUP62 antibody

As one of the FG-repeats containing nucleoporins, Nup62 is involved in nuclear transport, cell migration, virus infection, and cell cycle regulation. Nucleoporin Nup62 localizes at the central channel of the nuclear pore complex and is essential for nucleocytoplasmic transport. Through its FG-repeat domain, Nup62 regulates nuclear pore permeability and binds nuclear transport receptors. Nucleoporin Nup62 plays a novel role in centrosome integrity. Knockdown of Nup62 induced mitotic arrest in G 2/M phases and mitotic cell death. Depletion of Nup62 using RNA interference results in defective centrosome segregation and centriole maturation during the G 2 phase. Moreover, Nup62 depletion in human cells leads to the appearance of multinucleated cells and induces the formation of multipolar centrosomes, centriole synthesis defects, dramatic spindle orientation defects, and centrosome component rearrangements that impair cell bi-polarity.

References

Wu Z, et al. (2016) Nup62, associated with spindle microtubule rather than spindle matrix, is involved in chromosome alignment and spindle assembly during mitosis. Cell Biol Int 40 (9): 968-975.Hubert T, et al. (2009) Exo70-mediated recruitment of nucleoporin nup62 at the leading edge of migrating cells is required for cell migration. Traffic 10 (9): 1257-1271.Hashizume C, et al. (2013) Nucleoporin nup62 maintains centrosome homeostasis. Cell Cycle 12 (24): 3804-3816.