IF (Immunofluorescence)
(Immunofluorescence staining of NUSAP1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NUSAP1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Rabbit anti-Human NUSAP1 Polyclonal Antibody | anti-NUSAP1 antibody
Anti-NUSAP1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
NUSAP1, Antibody; Anti-NUSAP1 Antibody, Rabbit Polyclonal; NUSAP1 Antibody, Rabbit PAb, Antigen Affinity Purified; nucleolar and spindle associated protein 1; Anti-ANKT Antibody; Anti-BM037 Antibody; Anti-LNP Antibody; Anti-NUSAP Antibody; Anti-PRO0310p1 Antibody; Anti-Q0310 Antibody; Anti-SAPL Antibody; anti-NUSAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NUSAP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-NUSAP1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
E Coli-derived Human NUSAP1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human NUSAP1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of NUSAP1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NUSAP1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of NUSAP1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NUSAP1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IHC (Immunohistochemistry)
(Immunochemical staining of human NUSAP1 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human NUSAP1 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-NUSAP1 antibody
Nucleolar and spindle-associated protein (NUSAP1) is a microtubule and chromatin-binding protein that stabilizes microtubules to prevent depolymerization, maintains spindle integrity. NUSAP1 could cross-link spindles into aster-like structures, networks, and fibers. NUSAP1 is an indispensable mitotic regulator. NUSAP1 is a microtubule-associated protein involved in mitosis. Aberrant NUSAP1 expression is associated with perturbed mitosis and tumorigenesis. It has also been found to plays essential roles in diverse biological processes, especially in cancer biology. Nucleolar and spindle-associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer treatment.
References
Qian Z, et al. (2018) Prognostic value of nusap1 in progression and expansion of glioblastoma multiforme. J Neurooncol 140 (2): 199-208.Liu Z, et al. (2018) High nusap1 expression predicts poor prognosis in colon cancer. Pathol Res Pract 214 (7): 968-973.Chen Y, et al. (2020) Microrna-769-5p suppresses cell growth and migration via targeting nusap1 in bladder cancer. J Clin Lab Anal 34 (5): e23193.
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Product Notes
The NUSAP1 (Catalog #AAA256836) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NUSAP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUSAP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NUSAP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUSAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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