WB (Western Blot)
(Detection of human and mouse NXF1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), Jurkat (50ug), and mouse NIH 3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-NXF1 antibody AAA212371 (lot AAA212371-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse NXF1 Polyclonal Antibody | anti-NXF1 antibody
Rabbit anti-NXF1 Antibody, Affinity Purified
Gene Names
NXF1; TAP; MEX67
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NXF1, Antibody; Rabbit anti-NXF1 Antibody, Affinity Purified; tip associating protein; tip-associated protein; Tip-associating protein; nuclear RNA export factor 1; MEX67; mRNA export factor TAP; TAP; Nuclear RNA export factor 1; anti-NXF1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NXF1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 569 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NXF1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), Jurkat (50ug), and mouse NIH 3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-NXF1 antibody AAA212371 (lot AAA212371-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse NXF1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), Jurkat (50ug), and mouse NIH 3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-NXF1 antibody AAA212371 (lot AAA212371-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NXF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-NXF1 antibody AAA212371 (lot AAA212371-1) used for IP at 6 ug/mg lysate. NXF1 was also immunoprecipitated by rabbit anti-NFX1 antibodies and For blotting immunoprecipitated NXF1, AAA212371 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human NXF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-NXF1 antibody AAA212371 (lot AAA212371-1) used for IP at 6 ug/mg lysate. NXF1 was also immunoprecipitated by rabbit anti-NFX1 antibodies and For blotting immunoprecipitated NXF1, AAA212371 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-NXF1 antibody
Nuclear RNA export factor 1 (NXF1) is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. NXF1 shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p [taken from NCBI Entrez Gene (Gene ID: 10482)].
Product Categories/Family for anti-NXF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,476 Da
NCBI Official Full Name
nuclear RNA export factor 1 isoform 1
NCBI Official Synonym Full Names
nuclear RNA export factor 1
NCBI Official Symbol
NXF1
NCBI Official Synonym Symbols
TAP; MEX67
NCBI Protein Information
nuclear RNA export factor 1
UniProt Protein Name
Nuclear RNA export factor 1
UniProt Gene Name
NXF1
UniProt Synonym Gene Names
TAP
UniProt Entry Name
NXF1_HUMAN
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Product Notes
The NXF1 nxf1 (Catalog #AAA212371) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NXF1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NXF1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NXF1 nxf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NXF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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