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WB (Western Blot)
(Western blot analysis of extracts from various samples, using NYREN18 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hepg2;)
Rabbit NYREN18 Polyclonal Antibody | anti-NYREN18 antibody
NYREN18 Antibody
Gene Names
NUB1; BS4; NUB1L; NYREN18
Reactivity
Human, Mouse, Monkey
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NYREN18, Antibody; NYREN18 Antibody; BS4; NEDD8 ultimate buster 1; Negative regulator of ubiquitin like proteins 1; Negative regulator of ubiquitin-like proteins 1; NUB1; NUB1_HUMAN; NUB1L; NY REN 18; NYREN18; Renal carcinoma antigen NY-REN-18; anti-NYREN18 antibody
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
NYREN18 antibody detects endogenous levels of total NYREN18
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
639
Applicable Applications for anti-NYREN18 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human NYREN18
Subcellular Location
Nucleus. Predominantly Nuclear.
Tissue Specificity
Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.
Predicted Cross Reactivity
Bovine, Sheep, Rabbit, Dog
Similarity
Bovine (83%), Sheep (83%), Rabbit (92%), Dog (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from various samples, using NYREN18 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hepg2;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using NYREN18 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hepg2;)
IF (Immunofluorescence)
(AAA320913 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA320913 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis on COS7 cell lysate using NYREN18 Antibody, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis on COS7 cell lysate using NYREN18 Antibody, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(AAA320913 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA320913 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NYREN18 antibody
Description: NYREN18 Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8 and its conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2. Induced by beta and gamma interferons. Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.
Function: Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
Subunit Structure: Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Isoform 1 binds to NEDD8 more efficiently than isoform 2. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates.
Function: Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
Subunit Structure: Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Isoform 1 binds to NEDD8 more efficiently than isoform 2. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 70 kDa
Predicted: 71 kDa
Predicted: 71 kDa
NCBI Official Full Name
NEDD8 ultimate buster 1 isoform 1
NCBI Official Synonym Full Names
negative regulator of ubiquitin like proteins 1
NCBI Official Symbol
NUB1
NCBI Official Synonym Symbols
BS4; NUB1L; NYREN18
NCBI Protein Information
NEDD8 ultimate buster 1
UniProt Protein Name
NEDD8 ultimate buster 1
UniProt Gene Name
NUB1
UniProt Synonym Gene Names
NYREN18
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Product Notes
The NYREN18 nub1 (Catalog #AAA320913) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NYREN18 Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's NYREN18 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NYREN18 nub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NYREN18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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