IF (Immunofluorescence)
(Immunofluorescence staining of OBFC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human OBFC1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)
Rabbit anti-Human OBFC1 Polyclonal Antibody | anti-STN1 antibody
Anti-OBFC1 Antibody, Rabbit Polyclonal
Gene Names
OBFC1; STN1; AAF44; AAF-44; RPA-32; bA541N10.2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
OBFC1, Antibody; Anti-OBFC1 Antibody, Rabbit Polyclonal; OBFC1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-AAF-44 Antibody; Anti-AAF44 Antibody; Anti-bA541N10.2 Antibody; Anti-RP11-541N10.2 Antibody; Anti-RPA-32 Antibody; Anti-STN1 Antibody; anti-STN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human OBFC1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-STN1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
A synthetic peptide corresponding to the center region of the Human OBFC1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human OBFC1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of OBFC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human OBFC1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of OBFC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human OBFC1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)
IHC (Immunohistochemistry)
(Immunochemical staining of human OBFC1 in human vagina with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human OBFC1 in human vagina with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-STN1 antibody
The yeast homolog of OBFC1 is Stn1, which plays a critical role in telomere regulation. OBFC1/AAF44 can localize to telomeres in human cells and bind to telomeric single-stranded DNA in vitro. Overexpression of an OBFC1 mutant resulted in elongated telomeres in human cells, implicating OBFC1/AAF4 in telomere length regulation. OBFC1/AAF44 represents a new player in the telomere interactome for telomere maintenance.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,119 Da
NCBI Official Full Name
CST complex subunit STN1
NCBI Official Synonym Full Names
oligonucleotide/oligosaccharide-binding fold containing 1
NCBI Official Symbol
OBFC1
NCBI Official Synonym Symbols
STN1; AAF44; AAF-44; RPA-32; bA541N10.2
NCBI Protein Information
CST complex subunit STN1; alpha accessory factor 44; oligonucleotide/oligosaccharide-binding fold-containing protein 1; replication protein A 32 kDa subunit; suppressor of cdc thirteen homolog
UniProt Protein Name
CST complex subunit STN1
UniProt Gene Name
OBFC1
UniProt Synonym Gene Names
STN1
UniProt Entry Name
STN1_HUMAN
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Product Notes
The STN1 obfc1 (Catalog #AAA254617) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-OBFC1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OBFC1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the STN1 obfc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OBFC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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