Rabbit OCLN Polyclonal Antibody | anti-OCLN antibody

OCLN Antibody

Cat. Num. AAA10929

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: OCLN Antibody is affinity chromatography purified via peptide column.
• Synonyms: OCLN, Antibody; OCLN Antibody; OCLN; BLCPMG; Occludin; occludin; anti-OCLN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: OCLN Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 522

• Applicable Applications for anti-OCLN antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence)
• Application Notes: OCLN antibody can be used for detection of OCLN by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN.
• Buffer: OCLN Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: OCLN antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IHC (Immunohistchemistry)

(Immunohistochemistry of OCLN in mouse brain tissue with OCLN Antibody at 5 μg/mL.)

IF (Immunofluorescence)

(Immunofluorescence of OCLN in mouse brain tissue with OCLN antibody at 20 μg/mL.Red: OCLN Antibody (5191)Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of OCLN in human liver tissue with OCLN antibody at 20 μg/ml.Red: OCLN Antibody (5191)Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of OCLN in Human Liver tissue with OCLN antibody at 20 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemistry of OCLN in human liver tissue with OCLN antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 μg/mL.)

Related Product Information for anti-OCLN antibody

OCLN Antibody: Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 20987418
• NCBI GeneID: 4950
• UniProt Accession #: Q16625; Q5U1V4; Q8N6K1; B5BU70; D2DU64; D2DU65; D2IGC0; D2IGC1; E2CYV9
• Molecular Weight: 8,175 Da
• NCBI Official Full Name: OCLN protein
• UniProt Protein Name: Occludin
• UniProt Gene Name: OCLN
• UniProt Entry Name: OCLN_HUMAN

Frequently Asked Questions

What is OCLN and what role does it play in tight junctions?

OCLN is also known as occludin, is a protein found in tight junctions between cells. It helps seal gaps between neighboring cells to control what passes through tissues. This is important for maintaining strong barriers in organs like the intestine and brain.

Is OCLN a reliable marker for epithelial barrier integrity or cell–cell junctions?

Yes, OCLN is a reliable marker for epithelial barrier integrity and cell–cell junctions. Changes in occludin levels signal weakened or damaged tight junctions. Researchers use it to check how well cells connect and protect barriers.

How does OCLN differ from other tight junction proteins like ZO-1 or claudins?

OCLN is a membrane-spanning protein that directly seals cell junctions. Claudins form the main tight junction strands, while ZO-1 is a scaffold protein inside the cell that connects junction proteins to the cell structure.

Can the OCLN antibody detect protein in both human and mouse tissues?

Yes, the OCLN antibody can detect protein in both human and mouse tissues. This makes it useful for studying human samples as well as animal models commonly used in research experiments.

Is OCLN commonly studied in cancer, inflammation, or barrier dysfunction research?

Yes, OCLN is commonly studied in cancer, inflammation, or barrier dysfunction research. Changes in occludin levels are linked to tumour spread, inflammation, and leaky barriers in diseases affecting the gut, lungs, and brain.

Can this antibody be used for immunohistochemistry or flow cytometry staining?

Yes, this antibody can be used for both immunohistochemistry and flow cytometry to detect and study occluding expression in cells and tissue samples under suitable experimental conditions.

What are the recommended applications and working dilutions for OCLN Rabbit antibody?

The OCLN Rabbit antibody is recommended for Western blot, immunohistochemistry, immunofluorescence, and ELISA. Working dilutions vary by experiment and sample, so researchers follow the datasheet and optimize in their lab.