Application Data
(Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE OCT3/4 antibody diluted at 1:1000)
Rabbit anti-Human, Mouse Oct4 Polyclonal Antibody | anti-Oct4 antibody
Oct4 Antibody
Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Antibodies were purified by antigen-affinity chromatography.
Synonyms
Oct4, Antibody; Oct4 Antibody; MGC22487 antibody; OTF-3 antibody; OTF3 antibody; OTF4 antibody; POU5F1 antibody; POU-type homeodomain-containing DNA-binding protein antibody; octamer-binding protein 3 antibody; POU domain transcription factor OCT4 antibody; octamer-binding protein 4 an; anti-Oct4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total Oct4 protein.
Purity/Purification
Antibodies were purified by antigen-affinity chromatography.
Form/Format
Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Concentration
0.48 mg/ml (varies by lot)
Sequence Length
265
Applicable Applications for anti-Oct4 antibody
WB (Western Blot)
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant fragment corresponding to a region within amino acids 101 and 325 of OCT3/4.
Target Name
Oct4
Preparation and Storage
Store at -20 degree C
Application Data
(Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE OCT3/4 antibody diluted at 1:1000)
Application Data
(Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE OCT3/4 antibody diluted at 1:1000)
Application Data
(Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE diluted at 1:500)
Application Data
(Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE diluted at 1:500)
Related Product Information for anti-Oct4 antibody
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
Product Categories/Family for anti-Oct4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,085 Da
NCBI Official Full Name
POU domain, class 5, transcription factor 1 isoform 3
NCBI Official Synonym Full Names
POU class 5 homeobox 1
NCBI Official Symbol
POU5F1
NCBI Official Synonym Symbols
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
NCBI Protein Information
POU domain, class 5, transcription factor 1
UniProt Protein Name
POU domain, class 5, transcription factor 1
UniProt Gene Name
POU5F1
UniProt Synonym Gene Names
OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
UniProt Entry Name
PO5F1_HUMAN
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Product Notes
The Oct4 pou5f1 (Catalog #AAA307556) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Oct4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Oct4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the Oct4 pou5f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Oct4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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