WB (Western Blot)
(Western blot analysis of extracts of mouse lung tissue, using ODF2 antibody.)
Rabbit ODF2 Polyclonal Antibody | anti-ODF2 antibody
ODF2 Antibody
Gene Names
ODF2; CT134; ODF84; ODF2/1; ODF2/2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ODF2, Antibody; ODF2 Antibody; 84 kDa outer dense fiber protein; Cancer/testis antigen 134; Cenexin 1; Cenexin; Cenexin1 variant 1; CT134; FLJ44866; KKT 4; KKT4; MGC111096; MGC9034; ODF 2; ODF 84; odf2; ODF2/1; ODF2/2; ODF84; ODFP2_HUMAN; OTTHUMP00000022274; OTTHUMP00000022275; Outer dense fiber of sperm tail 2; Outer dense fiber of sperm tails 2; Outer dense fiber of sperm tails protein 2; Outer dense fiber of sperm tails, 84 kD; Outer dense fiber protein 2; Sperm outer dense fiber major protein 2; Sperm tail structural protein; Testis specific autoantigen; anti-ODF2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ODF2 antibody detects endogenous levels of ODF2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
824
Applicable Applications for anti-ODF2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ODF2.
Subcellular Location
Cytoplasm > Cytoskeleton > Centrosome. Cell Projection > Cilium. Cytoplasm > Cytoskeleton > Centrosome > Centriole. Cytoplasm > Cytoskeleton > Spindle Pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.
Tissue Specificity
Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.
Predicted Cross Reactivity
Pig, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of mouse lung tissue, using ODF2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of mouse lung tissue, using ODF2 antibody.)
IHC (Immunohiostchemistry)
(AAA322886 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA322886 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2, using ODF2 antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2, using ODF2 antibody. Lane 1 was treated with the blocking peptide.)
Related Product Information for anti-ODF2 antibody
Function: Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
Subunit Structure: Self-associates. Associates with microtubules and forms a fibrillar structure partially linked to the microtubule network. Interacts via its C-terminus with PLK1 (PubMed:16966375). Interacts with ODF1 (By similarity). Interacts with MARK4; the interaction is required for localization of ODF2 to centrioles (PubMed:23400999).
Post-translational Modifications: Tyrosine phosphorylated.
Similarity: Belongs to the ODF2 family.
Subunit Structure: Self-associates. Associates with microtubules and forms a fibrillar structure partially linked to the microtubule network. Interacts via its C-terminus with PLK1 (PubMed:16966375). Interacts with ODF1 (By similarity). Interacts with MARK4; the interaction is required for localization of ODF2 to centrioles (PubMed:23400999).
Post-translational Modifications: Tyrosine phosphorylated.
Similarity: Belongs to the ODF2 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 67-100 kDa
Predicted: 96 kDa
Predicted: 96 kDa
NCBI Official Full Name
outer dense fiber protein 2 isoform 11
NCBI Official Synonym Full Names
outer dense fiber of sperm tails 2
NCBI Official Symbol
ODF2
NCBI Official Synonym Symbols
CT134; ODF84; ODF2/1; ODF2/2
NCBI Protein Information
outer dense fiber protein 2
UniProt Protein Name
Outer dense fiber protein 2
UniProt Gene Name
ODF2
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Product Notes
The ODF2 odf2 (Catalog #AAA322886) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ODF2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ODF2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ODF2 odf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ODF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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