FCM/FACS (Flow Cytometry)
(OGN Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit OGN Polyclonal Antibody | anti-OGN antibody
OGN Antibody (C-term)
Gene Names
OGN; OG; OIF; SLRR3A
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
OGN, Antibody; OGN Antibody (C-term); Mimecan; Osteoglycin; Osteoinductive factor; OIF; OGN; SLRR3A; anti-OGN antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This OGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-276 amino acids from the C-terminal region of human OGN.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
246-276
Applicable Applications for anti-OGN antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Induces bone formation in conjunction with TGF-beta-1 or TGF- beta-2.
Cellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Location
Bone.
Antigen Source
HUMAN
Tissue Location
Bone
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(OGN Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(OGN Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(OGN Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of OGN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(OGN Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of OGN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(All lanes : Anti-OGN Antibody (N-term K75) at 1:500 dilutionLane 1: CCRF-CEM whole cell lysateLane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-OGN Antibody (N-term K75) at 1:500 dilutionLane 1: CCRF-CEM whole cell lysateLane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-OGN antibody
This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). Alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq].
Product Categories/Family for anti-OGN antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Dimasi, D.P., et al. Mol. Vis. 16, 562-569 (2010) :
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Dimasi, D.P., et al. Mol. Vis. 16, 562-569 (2010) :
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
mimecan preproprotein
NCBI Official Synonym Full Names
osteoglycin
NCBI Official Symbol
OGN
NCBI Official Synonym Symbols
OG; OIF; SLRR3A
NCBI Protein Information
mimecan
UniProt Protein Name
Mimecan
UniProt Gene Name
OGN
UniProt Synonym Gene Names
OIF; SLRR3A; OIF
UniProt Entry Name
MIME_HUMAN
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Product Notes
The OGN ogn (Catalog #AAA284340) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 246-276. The OGN Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's OGN can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the OGN ogn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OGN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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