FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-OLFML2B antibody (AAA127892).Overlay histogram showing SiHa cells stained with AAA127892 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLFML2B Antibody (AAA127892, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Rabbit anti-Human OLFML2B Polyclonal Antibody | anti-OLFML2B antibody
Anti-OLFML2B Antibody Picoband
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
OLFML2B, Antibody; Anti-OLFML2B Antibody Picoband; Transmembrane protein 240; kelch repeat and BTB domain containing 2; Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489; anti-OLFML2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-OLFML2B antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human OLFML2B recombinant protein (Position: I23-N671).
Subcellular Localization
Cul3-RING ubiquitin ligase complex
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-OLFML2B antibody (AAA127892).Overlay histogram showing SiHa cells stained with AAA127892 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLFML2B Antibody (AAA127892, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-OLFML2B antibody (AAA127892).Overlay histogram showing SiHa cells stained with AAA127892 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLFML2B Antibody (AAA127892, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot)
(Figure 1. Western blot analysis of OLFML2B using anti-OLFML2B antibody (AAA127892).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLFML2B antigen affinity purified polyclonal antibody (#AAA127892) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OLFML2B at approximately 96 kDa. The expected band size for OLFML2B is at 84 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of OLFML2B using anti-OLFML2B antibody (AAA127892).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLFML2B antigen affinity purified polyclonal antibody (#AAA127892) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OLFML2B at approximately 96 kDa. The expected band size for OLFML2B is at 84 kDa.)
Related Product Information for anti-OLFML2B antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-OLFML2B antibody
References
1. Liu, J. , Liu, Z. , Zhang, X. , Gong, T. , & Yao, D. . (2019). Bioinformatic exploration of olfml2b overexpression in gastric cancer base on multiple analyzing tools. BMC Cancer, 19(1), 1-10. 2. Nolte, I. M. , Wallace, C. , Newhouse, S. J. , Waggott, D. , Fu, J. , & Soranzo, N. , et al. (2009). Common genetic variation near the phospholamban gene is associated with cardiac repolarisation: meta-analysis of three genome-wide association studies. PLoS ONE, 4(7), e6138.3. Reiner, A. , Beleza, S. , Franceschini, N. , Auer, P. , Robinson, J. , & Kooperberg, C. , et al. (2012). Genome-wide association and population genetic analysis of c-reactive protein in african american and hispanic american women. The American Journal of Human Genetics, 91(3), 502-512.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
olfactomedin-like protein 2B isoform 2
NCBI Official Synonym Full Names
olfactomedin-like 2B
NCBI Official Symbol
OLFML2B
NCBI Protein Information
olfactomedin-like protein 2B; photomedin-2
UniProt Protein Name
Olfactomedin-like protein 2B
UniProt Gene Name
OLFML2B
UniProt Entry Name
OLM2B_HUMAN
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Product Notes
The OLFML2B olfml2b (Catalog #AAA127892) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-OLFML2B Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OLFML2B can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the OLFML2B olfml2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OLFML2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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