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product-image-AAA291738_IHC11.jpg IHC (Immunohistochemisry) (Dilution: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ORP1 Polyclonal Antibody, Unconjugated(bs-17514R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

Rabbit ORP1 Polyclonal Antibody | anti-ORP1 antibody

ORP1 Polyclonal Antibody

Gene Names
OSBPL1A; ORP1; ORP-1; OSBPL1B
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ORP1, Antibody; ORP1 Polyclonal Antibody; Oxysterol-binding protein-related protein 1; ORP-1; OSBP-related protein 1; OSBPL1A; ORP1; OSBP8; OSBPL1; OSBPL1B; anti-ORP1 antibody
Ordering
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-ORP1 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Dilution: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ORP1 Polyclonal Antibody, Unconjugated(bs-17514R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

product-image-AAA291738_IHC11.jpg IHC (Immunohistochemisry) (Dilution: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-ORP1 Polyclonal Antibody, Unconjugated(bs-17514R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

IHC (Immunohiostchemistry)

(Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-HAVCR1 Polyclonal Antibody, Unconjugated(bs-17514R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

product-image-AAA291738_IHC13.jpg IHC (Immunohiostchemistry) (Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-HAVCR1 Polyclonal Antibody, Unconjugated(bs-17514R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

WB (Western Blot)

(Protein: brain(mouse) lystes at 40ug;Primary: Anti-ORP1 (bs-17514R) at 1:300;Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;ECL excitated the fluorescence;Predicted band size :108 kDObserved band size :108 kD)

product-image-AAA291738_WB15.jpg WB (Western Blot) (Protein: brain(mouse) lystes at 40ug;Primary: Anti-ORP1 (bs-17514R) at 1:300;Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;ECL excitated the fluorescence;Predicted band size :108 kDObserved band size :108 kD)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65,095 Da
NCBI Official Full Name
oxysterol-binding protein-related protein 1 isoform 3
NCBI Official Synonym Full Names
oxysterol binding protein-like 1A
NCBI Official Symbol
OSBPL1A
NCBI Official Synonym Symbols
ORP1; ORP-1; OSBPL1B
NCBI Protein Information
oxysterol-binding protein-related protein 1; OSBP-related protein 1; oxysterol binding protein-like 1B; oxysterol-binding protein-related protein 1 variant 1; oxysterol-binding protein-related protein 1 variant 2
UniProt Protein Name
Oxysterol-binding protein-related protein 1
UniProt Gene Name
OSBPL1A
UniProt Synonym Gene Names
ORP1; OSBP8; OSBPL1; OSBPL1B; ORP-1; OSBP-related protein 1
UniProt Entry Name
OSBL1_HUMAN

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Product Notes

The ORP1 osbpl1a (Catalog #AAA291738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ORP1 Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ORP1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ORP1 osbpl1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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