IF (Immunofluorescence)
(Immunofluorescence staining of OS9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human OS9 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human OS9/osteosarcoma amplified 9 Polyclonal Antibody | anti-OS9 antibody
Anti-OS9/osteosarcoma amplified 9 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
OS9/osteosarcoma amplified 9, Antibody; Anti-OS9/osteosarcoma amplified 9 Antibody, Rabbit Polyclonal; OS9/osteosarcoma amplified 9 Antibody, Rabbit PAb, Antigen Affinity Purified; osteosarcoma amplified 9, endoplasmic reticulum lectin; Anti-ERLEC2 Antibody; Anti-OS-9 Antibody; anti-OS9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human OS9/osteosarcoma amplified 9
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-OS9 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
E Coli-derived Human OS9/osteosarcoma amplified 9 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human OS9/osteosarcoma amplified 9 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of OS9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human OS9 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of OS9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human OS9 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human OS9 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human OS9 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human OS9 in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human OS9 in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-OS9 antibody
OS9 (OS9 Endoplasmic Reticulum Lectin) is a Protein Coding gene. The OS9 gene, located on 12q13.3-q14.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, zebrafish, and frog. OS9 is an ER-resident glycoprotein containing a mannose-6-phosphate receptor homology domain, which is also found in yeast and mammalian lectins involved in endoplasmic reticulum-associated degradation (ERAD). The OS9 protein plays an important role in ERAD and acts during Toll-like receptor-induced activation of myeloid cells. Diseases associated with OS9 include Submucous Uterine Fibroid and Amelogenesis Imperfecta, Hypoplastic/Hypomaturation, X-Linked 2. Among its related pathways are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Signaling by GPCR. 181 organisms have orthologs with the human gene OS9.
References
Sun L, et al. (2015) A novel role of os-9 in the maintenance of intestinal barrier function from hypoxia-induced injury via p38-dependent pathway. Int J Biol Sci 11 (6): 664-671.Huttner S, et al. (2012) Unraveling the function of arabidopsis thaliana os9 in the endoplasmic reticulum-associated degradation of glycoproteins. Plant Mol Biol 79 (1-2): 21-33.Hofmann S, et al. (2013) Genome-wide association analysis reveals 12q13.3-q14.1 as new risk locus for sarcoidosis. Eur Respir J 41 (4): 888-900.
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Product Notes
The OS9 (Catalog #AAA256029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-OS9/osteosarcoma amplified 9 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OS9/osteosarcoma amplified 9 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the OS9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OS9/osteosarcoma amplified 9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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