WB (Western Blot)
(Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsGSK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Rabbit anti-Oryza sativa OsGSK2 Polyclonal Antibody | anti-OsGSK2 antibody
OsGSK2 Rabbit pAb
Gene Names
LOC4327333; CKX2; Gn1a; OsCKX2; OsJ_00744; B1046G12.8
Reactivity
Oryza sativa
Applications
Western Blot
Purity
Affinity purification
Synonyms
OsGSK2, Antibody; OsGSK2 Rabbit pAb; OsGSK2; anti-OsGSK2 antibody
Host
Rabbit
Reactivity
Oryza sativa
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-OsGSK2 antibody
WB (Western Blot)
Positive Samples
leaves (before flower), leaves sheath (before flower)
Immunogen
A synthetic peptide of Oryza sativa OsGSK2.
Cellular Location
cytoplasm, nucleus
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsGSK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsGSK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Related Product Information for anti-OsGSK2 antibody
Serine-threonine kinase that acts as a negative regulator of brassinosteroid (BR) signaling. Phosphorylates DLT and BZR1, two positive regulators that mediates several BR responses. Phosphorylation of DLT and BZR1 inhibits their activities in BR signaling. Phosphorylates OFP8, a positive regulator of BR responses. Phosphorylated OFP8 shuttles from the nucleus to the cytoplasm where it is degraded by the proteasome. Phosphorylates the E3 ubiquitin-protein ligase PUB24, a negative regulator of BR signaling, which targets BZR1 and promotes its degradation via the 26S proteasome. Phosphorylation of PUB24 increases its stability. Phosphorylates the AP2-ERF transcription factor SMOS1, a positive regulator of BR signaling, which cooperatively functions in a transactivating complex with BZR1 to enhance the transcription of BR biosynthetic genes. Phosphorylation of SMOS1 leads to its degradation by an unknown mechanism.
Product Categories/Family for anti-OsGSK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,021 Da
NCBI Official Full Name
cytokinin dehydrogenase 2
NCBI Official Symbol
LOC4327333
NCBI Official Synonym Symbols
CKX2; Gn1a; OsCKX2; OsJ_00744; B1046G12.8
NCBI Protein Information
cytokinin dehydrogenase 2
UniProt Protein Name
Cytokinin dehydrogenase 2
UniProt Gene Name
CKX2
UniProt Synonym Gene Names
OsCKX2; Gn1a
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Product Notes
The OsGSK2 ckx2 (Catalog #AAA282243) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OsGSK2 Rabbit pAb reacts with Oryza sativa and may cross-react with other species as described in the data sheet. AAA Biotech's OsGSK2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the OsGSK2 ckx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OsGSK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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