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IHC (Immunohistochemistry)
(Osteonectin/SPARC Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human Osteonectin/SPARC Polyclonal Antibody | anti-SPARC antibody
Osteonectin/SPARC Rabbit anti-Human Polyclonal Antibody
Gene Names
SPARC; ON
Reactivity
Human
Applications
Immunohistochemistry
Purity
Immunoaffinity purified
Synonyms
Osteonectin/SPARC, Antibody; Osteonectin/SPARC Rabbit anti-Human Polyclonal Antibody; PathPlus Osteonectin/SPARC Antibody; SPARC; Basement-membrane protein 40; BM-40; Cysteine-rich protein; ON; Osteonectin; anti-SPARC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.1% Sodium Azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SPARC antibody
IHC (Immunohistochemistry)
Target
Human Osteonectin/SPARC
Immunogen
LP-11345, synthetic peptide
Conjugation
Unconjugated
Preparation and Storage
Aliquot and store undiluted at -20 degree C or below for up to 1 year. Can be stored undiluted at 4 degree C for up to 1 month. Avoid freeze-thaw cycles.
IHC (Immunohistochemistry)
(Osteonectin/SPARC Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Osteonectin/SPARC Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(Osteonectin/SPARC Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(Osteonectin/SPARC Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(Osteonectin/SPARC Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(Osteonectin/SPARC Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Osteonectin/SPARC Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Osteonectin/SPARC Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
Related Product Information for anti-SPARC antibody
SPARC antibody is an unconjugated rabbit polyclonal antibody to SPARC (Osteonectin) from human. Validated for IHC.
SPARC (secreted protein acidic and rich in cysteine, Osteonectin, BM-40) is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. Tumor growth is enhanced in mice lacking SPARC due to changes in the ECM that create a more permissive environment for tumor progression. Additionally, SPARC is overexpressed in breast, prostate, colorectal and other cancers, and high expression of SPARC is a marker of poor prognosis in rectal cancer. In immunohistochemistry of normal tissue, SPARC has cytoplasmic positivity in endothelial cells, megakaryocytes, fibroblasts, some glial cells, seminiferous ducts of the testes, and neurons in the brain.
SPARC (secreted protein acidic and rich in cysteine, Osteonectin, BM-40) is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. Tumor growth is enhanced in mice lacking SPARC due to changes in the ECM that create a more permissive environment for tumor progression. Additionally, SPARC is overexpressed in breast, prostate, colorectal and other cancers, and high expression of SPARC is a marker of poor prognosis in rectal cancer. In immunohistochemistry of normal tissue, SPARC has cytoplasmic positivity in endothelial cells, megakaryocytes, fibroblasts, some glial cells, seminiferous ducts of the testes, and neurons in the brain.
References
The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; Cancer Biomark. 2017;18(4):459-466, PMID: 28009327;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,632 Da
NCBI Official Full Name
SPARC
NCBI Official Synonym Full Names
secreted protein, acidic, cysteine-rich (osteonectin)
NCBI Official Symbol
SPARC
NCBI Official Synonym Symbols
ON
NCBI Protein Information
SPARC; BM-40; basement-membrane protein 40; cysteine-rich protein; osteonectin; secreted protein acidic and rich in cysteine
UniProt Protein Name
SPARC
UniProt Gene Name
SPARC
UniProt Synonym Gene Names
ON; BM-40; ON
UniProt Entry Name
SPRC_HUMAN
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Product Notes
The SPARC sparc (Catalog #AAA163142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Osteonectin/SPARC Rabbit anti-Human Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Osteonectin/SPARC can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SPARC sparc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteonectin/SPARC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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