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IHC (Immunohistochemistry)
(AAA322584 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse OTUB2 Polyclonal Antibody | anti-OTUB2 antibody
OTUB2 Antibody
Gene Names
OTUB2; OTB2; OTU2; C14orf137
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
OTUB2, Antibody; OTUB2 Antibody; C14orf137; Deubiquitinating enzyme OTUB2; FLJ21916; MGC3102; OTB2; OTU domain containing ubiquitin aldehyde binding protein 2; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; OTU2; Otub2; OTUB2_HUMAN; Otubain 2; Otubain-2; Ubiquitin specific processing protease OTUB2; Ubiquitin specific protease otubain 2; Ubiquitin thioesterase OTUB2; Ubiquitin-specific-processing protease OTUB2; anti-OTUB2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
OTUB2 antibody detects endogenous levels of total OTUB2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
234
Applicable Applications for anti-OTUB2 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human OTUB2
Tissue Specificity
Widely expressed. Expressed at higher level in brain.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (89%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(AAA322584 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA322584 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using OTUB2 Antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using OTUB2 Antibody. Lane 1 was treated with the blocking peptide.)
IF (Immunofluorescence)
(AAA322584 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA322584 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis OTUB2 using HepG2 whole cell lysates)
WB (Western Blot)
(Western blot analysis OTUB2 using HepG2 whole cell lysates)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using OTUB2 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hela;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using OTUB2 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hela;)
Related Product Information for anti-OTUB2 antibody
Function: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-, 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
Similarity: Belongs to the peptidase C65 family.
Similarity: Belongs to the peptidase C65 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 27 kDa
Predicted: 28 kDa
Predicted: 28 kDa
NCBI Official Full Name
ubiquitin thioesterase OTUB2
NCBI Official Synonym Full Names
OTU deubiquitinase, ubiquitin aldehyde binding 2
NCBI Official Symbol
OTUB2
NCBI Official Synonym Symbols
OTB2; OTU2; C14orf137
NCBI Protein Information
ubiquitin thioesterase OTUB2
UniProt Protein Name
Ubiquitin thioesterase OTUB2
UniProt Gene Name
OTUB2
UniProt Synonym Gene Names
C14orf137; OTB2; OTU2
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Product Notes
The OTUB2 otub2 (Catalog #AAA322584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OTUB2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's OTUB2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the OTUB2 otub2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OTUB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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