FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-OXER1 antibody (AAA127651).Overlay histogram showing U937 cells stained with AAA127651 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-OXER1 Antibody (AAA127651, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Rabbit anti-Human OXER1 Polyclonal Antibody | anti-OXER1 antibody
Anti-OXER1 Antibody Picoband
Gene Names
OXER1; GPCR; GPR170; TG1019
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
OXER1, Antibody; Anti-OXER1 Antibody Picoband; Protein NDRG3; ring finger protein 169; E3 ubiquitin-protein ligase RNF169; RING finger protein 169; RING-type E3 ubiquitin transferase RNF169; RNF169; KIAA1991; anti-OXER1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-OXER1 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human OXER1 recombinant protein (Position: M1-Q422).
Subcellular Localization
Nucleoplasm
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-OXER1 antibody (AAA127651).Overlay histogram showing U937 cells stained with AAA127651 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-OXER1 Antibody (AAA127651, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-OXER1 antibody (AAA127651).Overlay histogram showing U937 cells stained with AAA127651 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-OXER1 Antibody (AAA127651, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot)
(Figure 1. Western blot analysis of OXER1 using anti-OXER1 antibody (AAA127651).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human THP-1 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXER1 antigen affinity purified polyclonal antibody (#AAA127651) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OXER1 at approximately 43-46 kDa. The expected band size for OXER1 is at 43-46 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of OXER1 using anti-OXER1 antibody (AAA127651).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human THP-1 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXER1 antigen affinity purified polyclonal antibody (#AAA127651) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OXER1 at approximately 43-46 kDa. The expected band size for OXER1 is at 43-46 kDa.)
Related Product Information for anti-OXER1 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-OXER1 antibody
References
1. Dattilo, M., Neuman, I., Munoz, M., Maloberti, P., Cornejo Maciel, F. OxeR1 regulates angiotensin II and cAMP-stimulated steroid production in human H295R adrenocortical cells. Molec. Cell. Endocr. 408: 38-44, 20152. Enyedi, B., Kala, S., Nikolich-Zugich, T., Niethammer, P. Tissue damage detection by osmotic surveillance. Nature Cell Biol. 15: 1123-1130, 2013.3. Hosoi, T., Koguchi, Y., Sugikawa, E., Chikada, A., Ogawa, K., Tsuda, N., Suto, N., Tsunoda, S., Taniguchi, T., Ohnuki, T. Identification of a novel human eicosanoid receptor coupled to G(i/o). J. Biol. Chem. 277: 31459-31465, 2002.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,811 Da
NCBI Official Full Name
oxoeicosanoid receptor 1
NCBI Official Synonym Full Names
oxoeicosanoid (OXE) receptor 1
NCBI Official Symbol
OXER1
NCBI Official Synonym Symbols
GPCR; GPR170; TG1019
NCBI Protein Information
oxoeicosanoid receptor 1; G-protein coupled receptor 170; G-protein coupled receptor R527; G-protein coupled receptor TG1019; 5-oxo-ETE G-protein coupled receptor; 5-oxo-ETE acid G-protein-coupled receptor 1
UniProt Protein Name
Oxoeicosanoid receptor 1
UniProt Gene Name
OXER1
UniProt Synonym Gene Names
GPR170; TG1019
UniProt Entry Name
OXER1_HUMAN
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Product Notes
The OXER1 oxer1 (Catalog #AAA127651) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-OXER1 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OXER1 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the OXER1 oxer1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OXER1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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