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WB (Western Blot)
(Western blot analysis of p15 INK expression in HeLa cell extract.The lane on the left is treated with the antigen-specific peptide.)
Rabbit p15 INK Polyclonal Antibody | anti-CDKN2B antibody
p15 INK Antibody
Gene Names
CDKN2B; P15; MTS2; TP15; CDK4I; INK4B; p15INK4b
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
p15 INK, Antibody; p15 INK Antibody; CDK inhibitory protein; CDK4B Inhibitor; Cdkn2b; CDN2B_HUMAN; Cyclin dependent kinase 4 inhibitor B; Cyclin Dependent Kinase Inhibitor 2B; Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4; Cyclin dependent kinases 4 and 6 binding protein; Cyclin-dependent kinase 4 inhibitor B; INK4B; MTS 2; MTS-2; MTS2; Multiple tumor suppressor 2; Multiple Tumor Supressor 2; OTTHUMP00000021154; OTTHUMP00000021155; p14 CDK inhibitor; p14 INK4b; p14-INK4b; P15; p15 CDK inhibitor; p15 inhibits CDK4; p15 INK4b; p15-INK4b; p15INK4B; TP 15; TP15; anti-CDKN2B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
p15 INK antibody detects endogenous levels of total p15 INK
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
138
Applicable Applications for anti-CDKN2B antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human p15 INK
Tissue Specificity
Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.
Predicted Cross Reactivity
Bovine, Horse, Rabbit, Dog
Similarity
Bovine (100%), Horse (88%), Rabbit (94%), Dog (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of p15 INK expression in HeLa cell extract.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of p15 INK expression in HeLa cell extract.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts of various celllines, using p15 INK Antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various celllines, using p15 INK Antibody.)
IF (Immunofluorescence)
(AAA322115 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA322115 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA322115 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
IF (Immunofluorescence)
(AAA322115 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
IF (Immunofluorescence)
(AAA322115 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA322115 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-CDKN2B antibody
Description: Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
Subunit Structure: Heterodimer of CDKN2B with CDK4 or CDK6. Isoform 2 does not interact with CDK4 nor CDK6.
Similarity: Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Subunit Structure: Heterodimer of CDKN2B with CDK4 or CDK6. Isoform 2 does not interact with CDK4 nor CDK6.
Similarity: Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 15 kDa
Predicted: 15 kDa
Predicted: 15 kDa
NCBI Official Full Name
cyclin-dependent kinase 4 inhibitor B isoform 1
NCBI Official Synonym Full Names
cyclin dependent kinase inhibitor 2B
NCBI Official Symbol
CDKN2B
NCBI Official Synonym Symbols
P15; MTS2; TP15; CDK4I; INK4B; p15INK4b
NCBI Protein Information
cyclin-dependent kinase 4 inhibitor B
UniProt Protein Name
Cyclin-dependent kinase 4 inhibitor B
UniProt Gene Name
CDKN2B
UniProt Synonym Gene Names
MTS2; MTS-2; p15INK4B
Similar Products
Product Notes
The CDKN2B cdkn2b (Catalog #AAA322115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p15 INK Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p15 INK can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CDKN2B cdkn2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p15 INK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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