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FCM/FACS (Flow Cytometry)
(p21 (CDKN1A) Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human p21 (CDKN1A) Polyclonal Antibody | anti-CDKN1A antibody
p21 (CDKN1A) Antibody (C-term)
Gene Names
CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
p21 (CDKN1A), Antibody; p21 (CDKN1A) Antibody (C-term); Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21; CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; anti-CDKN1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This p21 (CDKN1A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 amino acids from the C-terminal region of human p21 (CDKN1A).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
134-164
Sequence Length
164
Applicable Applications for anti-CDKN1A antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(p21 (CDKN1A) Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(p21 (CDKN1A) Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of p21 (CDKN1A) Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of p21 (CDKN1A) Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(The anti-CDKN1A Pab is used in Western blot to detect CDKN1A in T-47D cell lysate.)
WB (Western Blot)
(The anti-CDKN1A Pab is used in Western blot to detect CDKN1A in T-47D cell lysate.)
Related Product Information for anti-CDKN1A antibody
CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
Product Categories/Family for anti-CDKN1A antibody
References
Fukuchi, K., et al., Biochim. Biophys. Acta 1642(3):163-171 (2003).
Frouin, I., et al., J. Biol. Chem. 278(41):39265-39268 (2003).
Dupont, J., et al., J. Biol. Chem. 278(39):37256-37264 (2003).
Di Padova, M., et al., J. Biol. Chem. 278(38):36496-36504 (2003).
Bai, Y.Q., et al., Oncogene 22(39):7942-7949 (2003).
Frouin, I., et al., J. Biol. Chem. 278(41):39265-39268 (2003).
Dupont, J., et al., J. Biol. Chem. 278(39):37256-37264 (2003).
Di Padova, M., et al., J. Biol. Chem. 278(38):36496-36504 (2003).
Bai, Y.Q., et al., Oncogene 22(39):7942-7949 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18119
NCBI Official Full Name
cyclin-dependent kinase inhibitor 1 isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
NCBI Official Symbol
CDKN1A
NCBI Official Synonym Symbols
P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
NCBI Protein Information
cyclin-dependent kinase inhibitor 1
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1
UniProt Gene Name
CDKN1A
UniProt Synonym Gene Names
CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6
UniProt Entry Name
CDN1A_HUMAN
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Product Notes
The CDKN1A cdkn1a (Catalog #AAA289189) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 134-164. The p21 (CDKN1A) Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p21 (CDKN1A) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CDKN1A cdkn1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p21 (CDKN1A), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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