IHC (Immunohistochemistry)
(AAA13663 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat P2RX7/P2X7 receptor Polyclonal Antibody | anti-P2RX7 antibody
Goat anti-P2RX7/P2X7 receptor Antibody
Gene Names
P2RX7; P2X7
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
P2RX7/P2X7 receptor, Antibody; Goat anti-P2RX7/P2X7 receptor Antibody; P2RX7; purinergic receptor P2X, ligand-gated ion channel, 7; MGC20089; P2X7; ATP receptor; P2X purinoceptor 7; P2X7 receptor; P2Z receptor; purinergic receptor P2X7; P2RX7 antibody; purinergic receptor P2X; ligand-gated ion channel; 7 antibody; MGC20089 antibody; P2X7 antibody; ATP receptor antibody; P2X purinoceptor 7 antibody; P2X7 receptor antibody; P2Z receptor antibody; purinergic receptor P2X7 antibody; P2RX7 / P2X7 receptor; anti-P2RX7 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
0.5 mg/mL (varies by lot)
Sequence Length
595
Applicable Applications for anti-P2RX7 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 37kDa and 70kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kDa according to NP_002553.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Brain Cortex shows membranous staining of cell bodies and processes. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml.
Immunofluorescence (IF): This antibody has been succesfully used in IF on Mouse: Thaler R et al. (2015) PMID: 25491310.
Western blot: Approx 37kDa and 70kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kDa according to NP_002553.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Brain Cortex shows membranous staining of cell bodies and processes. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml.
Immunofluorescence (IF): This antibody has been succesfully used in IF on Mouse: Thaler R et al. (2015) PMID: 25491310.
Immunogen
Peptide with sequence YETNKVTRIQSMNY-C, from the N Terminus of the protein sequence according to NP_002553.2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IHC (Immunohistochemistry)
(AAA13663 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
(AAA13663 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
WB (Western Blot)
(Staining (0.3ug/ml) of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(Staining (0.3ug/ml) of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
P2X purinoceptor 7
NCBI Official Synonym Full Names
purinergic receptor P2X 7
NCBI Official Symbol
P2RX7
NCBI Official Synonym Symbols
P2X7
NCBI Protein Information
P2X purinoceptor 7
UniProt Protein Name
P2X purinoceptor 7
UniProt Gene Name
P2RX7
UniProt Synonym Gene Names
P2X7
UniProt Entry Name
P2RX7_HUMAN
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Product Notes
The P2RX7 p2rx7 (Catalog #AAA13663) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-P2RX7/P2X7 receptor Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's P2RX7/P2X7 receptor can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 37kDa and 70kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kDa according to NP_002553.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Brain Cortex shows membranous staining of cell bodies and processes. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml. Immunofluorescence (IF): This antibody has been succesfully used in IF on Mouse: Thaler R et al. (2015) PMID: 25491310. Researchers should empirically determine the suitability of the P2RX7 p2rx7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P2RX7/P2X7 receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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