IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Immunofluorescent analysis of P2Y4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Rabbit P2Y4 Polyclonal Antibody | anti-P2Y4 antibody
Anti-P2Y4 Antibody
Gene Names
P2RY4; NRU; P2P; UNR; P2Y4
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Synonyms
P2Y4, Antibody; Anti-P2Y4 Antibody; NRU; P2Y purinoceptor 4; P2Y4; P2P; Uridine nucleotide receptor; UNR; anti-P2Y4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Applicable Applications for anti-P2Y4 antibody
IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Immunofluorescent analysis of P2Y4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Immunofluorescent analysis of P2Y4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Western blot analysis of P2Y4 expression in Hela (A), H460 (B), mouse lung (C), mouse brain (D), mouse kidney (E), rat kidney (F) whole cell lysates.)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Western blot analysis of P2Y4 expression in Hela (A), H460 (B), mouse lung (C), mouse brain (D), mouse kidney (E), rat kidney (F) whole cell lysates.)
Related Product Information for anti-P2Y4 antibody
Rabbit polyclonal antibody to P2Y4
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human P2Y4. The exact sequence is proprietary.
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human P2Y4. The exact sequence is proprietary.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,963 Da
NCBI Official Full Name
P2Y purinoceptor 4
NCBI Official Synonym Full Names
pyrimidinergic receptor P2Y, G-protein coupled, 4
NCBI Official Symbol
P2RY4
NCBI Official Synonym Symbols
NRU; P2P; UNR; P2Y4
NCBI Protein Information
P2Y purinoceptor 4; uridine nucleotide receptor
UniProt Protein Name
P2Y purinoceptor 4
UniProt Gene Name
P2RY4
UniProt Synonym Gene Names
NRU; P2Y4; UNR
UniProt Entry Name
P2RY4_HUMAN
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Product Notes
The P2Y4 p2ry4 (Catalog #AAA292840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-P2Y4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's P2Y4 can be used in a range of immunoassay formats including, but not limited to, IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the P2Y4 p2ry4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P2Y4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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