WB (Western Blot)
(Detection of human p300 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody AAA210904 (lot AAA210904-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human p300 Polyclonal Antibody | anti-p300 antibody
Rabbit anti-p300 Antibody, Affinity Purified
Gene Names
EP300; p300; KAT3B; RSTS2
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
p300, Antibody; Rabbit anti-p300 Antibody, Affinity Purified; p300; RSTS2; p300 HAT; KAT3B; histone crotonyltransferase p300; histone butyryltransferase p300; histone acetyltransferase p300; E1A-binding protein, 300kD; E1A-associated protein p300; protein propionyltransferase p300; Histone acetyltransferase p300; E1A binding protein p300; anti-p300 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-p300 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1000 and 1050
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human p300 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody AAA210904 (lot AAA210904-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human p300 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody AAA210904 (lot AAA210904-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody AAA210904 (lot AAA210904-2) used for IP at 6 ug/mg lysate. p300 was also immunoprecipitated by a previous lot (lot AAA210904-1) of this antibody. For blotting immunoprecipitated p300, AAA210904-2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody AAA210904 (lot AAA210904-2) used for IP at 6 ug/mg lysate. p300 was also immunoprecipitated by a previous lot (lot AAA210904-1) of this antibody. For blotting immunoprecipitated p300, AAA210904-2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 (Cat. No. AAA210904 Lot2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 (Cat. No. AAA210904 Lot2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-p300 antibody
By sequence similarity p300 is related to CBP (CREB-binding protein) and functions as a histone acetyltransferase that can regulate transcription by influencing chromatin organization. P300 has been demonstrated to bind to phosphorylated CREB to mediate cAMP-gene regulation. P300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha).
Product Categories/Family for anti-p300 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
264,161 Da
NCBI Official Full Name
histone acetyltransferase p300
NCBI Official Synonym Full Names
E1A binding protein p300
NCBI Official Symbol
EP300
NCBI Official Synonym Symbols
p300; KAT3B; RSTS2
NCBI Protein Information
histone acetyltransferase p300; p300 HAT; E1A-binding protein, 300kD; E1A-associated protein p300
UniProt Protein Name
Histone acetyltransferase p300
UniProt Gene Name
EP300
UniProt Synonym Gene Names
P300; p300 HAT
UniProt Entry Name
EP300_HUMAN
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Product Notes
The p300 ep300 (Catalog #AAA210904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-p300 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p300 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the p300 ep300 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p300, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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