IF (Immunofluorescence)
(Immunofluorescent analysis of CDK5R1 p35 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Rabbit p35 Polyclonal Antibody | anti-CDK5R1 antibody
Anti-p35 Antibody
Gene Names
CDK5R1; p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
Reactivity
Human, Mouse, Rat, Bovine, Zebrafish
Applications
Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
p35, Antibody; Anti-p35 Antibody; CDK5R; NCK5A; Cyclin-dependent kinase 5 activator 1; CDK5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit; anti-CDK5R1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Zebrafish
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of CDK5R1 p35 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
307
Applicable Applications for anti-CDK5R1 antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p35. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of CDK5R1 p35 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of CDK5R1 p35 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IHC (Immunohiostchemistry)
IHC (Immunohiostchemistry)
WB (Western Blot)
(Western blot analysis of CDK5R1 p35 expression in U87MG (A), EC9706 (B), SHSY5Y (C) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of CDK5R1 p35 expression in U87MG (A), EC9706 (B), SHSY5Y (C) whole cell lysates.)
Related Product Information for anti-CDK5R1 antibody
Rabbit polyclonal antibody to CDK5R1 p35
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cyclin-dependent kinase 5 activator 1
NCBI Official Synonym Full Names
cyclin-dependent kinase 5, regulatory subunit 1 (p35)
NCBI Official Symbol
CDK5R1
NCBI Official Synonym Symbols
p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
NCBI Protein Information
cyclin-dependent kinase 5 activator 1; CDK5 activator 1; neuronal CDK5 activator; TPKII regulatory subunit; regulatory partner for CDK5 kinase; tau protein kinase II 23kDa subunit; cyclin-dependent kinase 5 regulatory subunit 1
UniProt Protein Name
Cyclin-dependent kinase 5 activator 1
UniProt Gene Name
CDK5R1
UniProt Synonym Gene Names
CDK5R; NCK5A; CDK5 activator 1; p35; p25; p23
UniProt Entry Name
CD5R1_HUMAN
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Product Notes
The CDK5R1 cdk5r1 (Catalog #AAA105106) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p35 Antibody reacts with Human, Mouse, Rat, Bovine, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's p35 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CDK5R1 cdk5r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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