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product-image-AAA293346_ELISA10.jpg ELISA (Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)Direct ELISA antibody dose-response curve using Anti-p38 (pY182) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.)

Rabbit p38 Polyclonal Antibody | anti-p38 antibody

Anti-p38 (pY182) Antibody

Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat, Zebrafish, Drosophila
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Synonyms
p38, Antibody; Anti-p38 (pY182) Antibody; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a; anti-p38 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish, Drosophila
Clonality
Polyclonal
Applicable Applications for anti-p38 antibody
IHC (Immunohistochemistry), IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

ELISA

(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)Direct ELISA antibody dose-response curve using Anti-p38 (pY182) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.)

product-image-AAA293346_ELISA10.jpg ELISA (Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)Direct ELISA antibody dose-response curve using Anti-p38 (pY182) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.)

IF (Immunofluorescence)

(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500))

product-image-AAA293346_IF11.jpg IF (Immunofluorescence) (Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500))

IHC (Immunohiostchemistry)

(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)Immunohistochemical analysis of p38 (pY182) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

product-image-AAA293346_IHC13.jpg IHC (Immunohiostchemistry) (Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)Immunohistochemical analysis of p38 (pY182) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot)

(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)Western blot analysis of p38 (pY182) expression in PC3 (A), MCF7 (B), Raw264.7 (C), mouse muscle (D), rat muscle (E) whole cell lysates.)

product-image-AAA293346_WB15.jpg WB (Western Blot) (Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)Western blot analysis of p38 (pY182) expression in PC3 (A), MCF7 (B), Raw264.7 (C), mouse muscle (D), rat muscle (E) whole cell lysates.)
Related Product Information for anti-p38 antibody
Rabbit polyclonal antibody to p38 (pY182)
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p38 (pY182). The exact sequence is proprietary.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,293 Da
NCBI Official Full Name
mitogen-activated protein kinase 14 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 14
NCBI Official Symbol
MAPK14
NCBI Official Synonym Symbols
RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
NCBI Protein Information
mitogen-activated protein kinase 14; MAP kinase 14; p38alpha Exip; p38 MAP kinase; MAP kinase Mxi2; MAP kinase p38 alpha; CSAID-binding protein; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; p38 mitogen activated p
UniProt Protein Name
Mitogen-activated protein kinase 14
UniProt Gene Name
MAPK14
UniProt Synonym Gene Names
CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; MAP kinase 14; MAPK 14; CSAID-binding protein; CSBP; MAP kinase p38 alpha; SAPK2a
UniProt Entry Name
MK14_HUMAN

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Product Notes

The p38 mapk14 (Catalog #AAA293346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p38 (pY182) Antibody reacts with Human, Mouse, Rat, Zebrafish, Drosophila and may cross-react with other species as described in the data sheet. AAA Biotech's p38 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the p38 mapk14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p38, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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