Rabbit p40 (deltaNp63) Polyclonal Antibody | anti-TP63 antibody

p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) Rabbit Polyclonal Antibody

Cat. Num. AAA13805

• Gene Names: TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
• Reactivity: Human, Mouse, Rat, Cow
• Applications: Western Blot, Immunohistochemistry
• Synonyms: p40 (deltaNp63), Antibody; p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) Rabbit Polyclonal Antibody; deltaNp63, TP63; anti-TP63 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Cow
• Clonality: Polyclonal
• Isotype: IgG/kappa
• Form/Format: 200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Sequence Length: 555

• Applicable Applications for anti-TP63 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: IHC: 1-2ug/ml 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.; WB: 2-4ug/ml
• Cellular Localization: Nucleus
• Immunogen: A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40
• Research Areas: Apoptosis, Basal Cell Marker, Cancer, Nuclear Marker, Stem Cell Differentiation, Transcription Factors
• Positive Control: HEK293 cells. Prostate Carcinoma or Lung Squamous Cell Carcinoma.
• Specificity Note: p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis of Purified p40 Rabbit Polyclonal Antibody.)

WB (Western Blot)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Prostate Carcinoma stained with p40 Rabbit Polyclonal Antibody.)

References

Bishop, JA et. al. Modern Pathology 25 : 405-4152. Scagliotti G et. al. J Thorac Oncol 6:64-70. 3. Kargi A et. al. Appl Immunohistochem Mol Morphol 15:415-420

NCBI and Uniprot Product Information

• NCBI GI #: 169234657
• NCBI GeneID: 8626
• NCBI Accession #: NP_001108450.1
• NCBI GenBank Nucleotide #: NM_001114978.1
• UniProt Accession #: Q9H3D4; O75080; O75195; O75922; O76078; Q6VEG2; Q6VEG3; Q6VEG4; Q6VFJ1; Q6VFJ2; Q6VFJ3; Q6VH20
• Molecular Weight: 40kDa
• NCBI Official Full Name: tumor protein 63 isoform 2
• NCBI Official Synonym Full Names: tumor protein p63
• NCBI Official Symbol: TP63
• NCBI Official Synonym Symbols: AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
• NCBI Protein Information: tumor protein 63
• UniProt Protein Name: Tumor protein 63
• UniProt Gene Name: TP63
• UniProt Synonym Gene Names: KET; P63; P73H; P73L; TP73L; p63; CUSP; TP63; p73L
• UniProt Entry Name: P63_HUMAN