IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, using Neutrophil Cytosol Factor 1 (Ab-304) antibody.)
Rabbit anti-Human p47 phox Polyclonal Antibody | anti-NCF1 antibody
p47 phox Antibody (Ab-304)
Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
p47 phox, Antibody; p47 phox Antibody (Ab-304); Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1; NOXO2; SH3PXD1A; anti-NCF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Positions
295-330
Sequence Length
390
Applicable Applications for anti-NCF1 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, using Neutrophil Cytosol Factor 1 (Ab-304) antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, using Neutrophil Cytosol Factor 1 (Ab-304) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells (Lane 1) and Jurket cells (Lane 2), using Neutrophil Cytosol Factor 1 (Ab-304) Antibody. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells (Lane 1) and Jurket cells (Lane 2), using Neutrophil Cytosol Factor 1 (Ab-304) Antibody. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of lysates from Ramos,RajI cell line (from left to right),using p47 phox Antibody (Ab-304)(B1160). B1160 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Ramos,RajI cell line (from left to right),using p47 phox Antibody (Ab-304)(B1160). B1160 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Related Product Information for anti-NCF1 antibody
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Product Categories/Family for anti-NCF1 antibody
References
Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989).
Lomax K.J.,et al.Science 245:409-412(1989).
Rodaway A.R.F.,et al.Mol. Cell. Biol. 10:5388-5396(1990).
Gorlach A.,et al.J. Clin. Invest. 100:1907-1918(1997).
Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989).
Lomax K.J.,et al.Science 245:409-412(1989).
Rodaway A.R.F.,et al.Mol. Cell. Biol. 10:5388-5396(1990).
Gorlach A.,et al.J. Clin. Invest. 100:1907-1918(1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44652
NCBI Official Full Name
neutrophil cytosol factor 1
NCBI Official Synonym Full Names
neutrophil cytosolic factor 1
NCBI Official Symbol
NCF1
NCBI Official Synonym Symbols
NCF1A; NOXO2; p47phox; SH3PXD1A
NCBI Protein Information
neutrophil cytosol factor 1
UniProt Protein Name
Neutrophil cytosol factor 1
UniProt Gene Name
NCF1
UniProt Synonym Gene Names
NOXO2; SH3PXD1A; NCF-1
UniProt Entry Name
NCF1_HUMAN
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Product Notes
The NCF1 ncf1 (Catalog #AAA286855) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 295-330. The p47 phox Antibody (Ab-304) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p47 phox can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NCF1 ncf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p47 phox, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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