WB (Western Blot)
(Western blot analysis of Phospho-p56 Dok-2 (Tyr299) expression in various lysates)
Rabbit anti-Human, Mouse p56 Dok-2 Polyclonal Antibody | anti-DOK2 antibody
Phospho-p56 Dok-2 (Tyr299) Antibody
Gene Names
DOK2; p56DOK; p56dok-2
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
p56 Dok-2, Antibody; Phospho-p56 Dok-2 (Tyr299) Antibody; Docking protein 2 56kDa; Docking protein 2; DOK 2; DOK R; DOK2; DOK2_HUMAN; Downstream of tyrosine kinase 2; OTTHUMP00000161710; OTTHUMP00000224923; p56(dok 2); p56(dok-2); p56dok 2; p56DOK; p56dok2; anti-DOK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-p56 Dok-2 (Tyr299) antibody detects endogenous levels of p56 Dok-2 only when phosphorylated at Tyrosine 299
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
412
Applicable Applications for anti-DOK2 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human p56 Dok-2 around the phosphorylation site of Tyrosine 299
Tissue Specificity
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
Predicted Cross Reactivity
Pig, Bovine, Horse, Dog, Xenopus
Similarity
Pig (91%), Bovine (91%), Horse (91%), Dog (91%), Xenopus (89%)
Conjugation
Unconjugated
Epitope
Phospho Tyr299
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-p56 Dok-2 (Tyr299) expression in various lysates)
WB (Western Blot)
(Western blot analysis of Phospho-p56 Dok-2 (Tyr299) expression in various lysates)
WB (Western Blot)
(Western blot analysis of p56 Dok-2 phosphorylation expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of p56 Dok-2 phosphorylation expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-DOK2 antibody
Description: The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML.
Function: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).
Subunit Structure: Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).
Post-translational Modifications: On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).
Similarity: PTB domain mediates receptor interaction. Belongs to the DOK family. Type A subfamily.
Function: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).
Subunit Structure: Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).
Post-translational Modifications: On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).
Similarity: PTB domain mediates receptor interaction. Belongs to the DOK family. Type A subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 56 kDa
Predicted: 46 kDa
Predicted: 46 kDa
NCBI Official Full Name
docking protein 2 isoform a
NCBI Official Synonym Full Names
docking protein 2
NCBI Official Symbol
DOK2
NCBI Official Synonym Symbols
p56DOK; p56dok-2
NCBI Protein Information
docking protein 2
UniProt Protein Name
Docking protein 2
UniProt Gene Name
DOK2
Similar Products
Product Notes
The DOK2 dok2 (Catalog #AAA321434) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-p56 Dok-2 (Tyr299) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p56 Dok-2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DOK2 dok2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p56 Dok-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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