WB (Western Blot)
(Detection of human p66alpha by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, HeLa, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p66alpha antibody (AAA211853 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse p66alpha Polyclonal Antibody | anti-p66alpha antibody
Rabbit anti-p66alpha Antibody, Affinity Purified
Gene Names
GATAD2A; p66alpha
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
p66alpha, Antibody; Rabbit anti-p66alpha Antibody, Affinity Purified; transcriptional repressor p66-alpha; p66alpha; GATA zinc finger domain-containing protein 2A; Hp66alpha; Transcriptional repressor p66-alpha; GATA zinc finger domain containing 2A; anti-p66alpha antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-p66alpha antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 583 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human p66alpha by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, HeLa, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p66alpha antibody (AAA211853 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human p66alpha by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, HeLa, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p66alpha antibody (AAA211853 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse p66alpha by western blot. Samples: Whole cell lysate (50 ug) from A20, WEHI-231, F9, and 70Z/3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p66alpha antibody (AAA211853 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse p66alpha by western blot. Samples: Whole cell lysate (50 ug) from A20, WEHI-231, F9, and 70Z/3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p66alpha antibody (AAA211853 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human p66alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p66alpha antibody (AAA211853 lot 3) used for IP at 6 ug per reaction. p66alpha was also immunoprecipitated by a previous lot of this antibody (AAA211853 lot 2) and a second antibody against a different epitope of p66alpha . For blotting immunoprecipitated p66alpha, AAA211853 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human p66alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p66alpha antibody (AAA211853 lot 3) used for IP at 6 ug per reaction. p66alpha was also immunoprecipitated by a previous lot of this antibody (AAA211853 lot 2) and a second antibody against a different epitope of p66alpha . For blotting immunoprecipitated p66alpha, AAA211853 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-p66alpha antibody
p66alpha and p66beta are human orthologs of the xenopus p66 subunit of the Mi-2/NuRD complex. The Mi-2/NuRD complex is a multi-subunit complex that mediates gene repression via processes involving chromatin remodeling and histone deacetylation. p66 alpha and beta were identified in a two-hybrid screen using the methyl-CpG-binding domain protein, MBD2, as bait. It has been demonstrated that p66alpha and p66beta mediate the interaction between MBD2 and histones.
Product Categories/Family for anti-p66alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,134 Da
NCBI Official Full Name
Homo sapiens GATA zinc finger domain containing 2A (GATAD2A), transcript variant 2, mRNA
NCBI Official Synonym Full Names
GATA zinc finger domain containing 2A
NCBI Official Symbol
GATAD2A
NCBI Official Synonym Symbols
p66alpha
NCBI Protein Information
transcriptional repressor p66-alpha; GATA zinc finger domain-containing protein 2A
UniProt Protein Name
Transcriptional repressor p66-alpha
UniProt Gene Name
GATAD2A
UniProt Synonym Gene Names
Hp66alpha
UniProt Entry Name
P66A_HUMAN
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Product Notes
The p66alpha gatad2a (Catalog #AAA211853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-p66alpha Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p66alpha can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the p66alpha gatad2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p66alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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